https://eln.excellgen.com/index.php?action=history&feed=atom&title=1422-pET28b.Amp 1422-pET28b.Amp - Revision history 2026-10-18T08:18:05Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1422-pET28b.Amp&diff=6571&oldid=prev Paul.xu at 19:43, 17 October 2012 2012-10-17T19:43:42Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1422-pET28b.Amp&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1422-pET28b.Amp <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-10-11---2012-10-17<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1422&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === subcloning ===<br /> <br /> [[Image:1422+1423 clone 10-10-2012.JPG]][[Image:1422 vector 10-11-2012.JPG]]&lt;br&gt; <br /> <br /> digest pET30b-Amp(lane1) with Alwn1 + Dra III--&amp;gt; insert&lt;br&gt;digest pET28b-Amp(lane2) with Alwn1 +DraIII--&amp;gt; vector&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2012-10-16<br /> <br /> Make miniprep and digest with Alwn1 +DraIII&lt;br&gt; <br /> <br /> [[Image:1422_digest_detect_10-17-2012.JPG]]<br /> <br /> &lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&amp;amp;amp;lt;br&amp;amp;amp;gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &amp;amp;amp;lt;br&amp;amp;amp;gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Category:Cloning]]</div> Paul.xu