Paul.xu at 12:59, 22 October 2012

2012-10-22T12:59:27Z

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== '''Cloning of 1429-pT7A3-MBP''' ==

'''Purpose:''' Cloning of 1429-pT7A3-MBP

'''Date: '''2012-10-18---2012-10-20

'''Notebook:0'''

'''Page:'''

'''Clone ID:1429'''

'''Experimenters:''' yingying

== '''Results''' ==

=== subcloning ===

[[Image:1095+1428+1429 clone 10-17-2012.JPG]][[Image:1429 clone 10-17-2012.JPG]] 

digest 1279 pT7A3 (v)with Xba1 + EcoR1---> vector digest pDB.His.MBP or pDB.His.MBP.amp(lane3,4,5) with Xba1 + EcoR1--->insert ligate and transform 

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=== Colony Screening ===

Date: 2012-10-20

Make miniprep and digest with Xba1 + EcoR1 

[[Image:1428_1429_1026_1416digest.JPG]]

clone1,2,3,4,5 right

== '''Result intepretation/Summary''' ==

*&amp;lt;br&amp;gt;

== '''Conclusion''' ==

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[[Category:Cloning]]

1429-pT7A3-MBP - Revision history

Paul.xu at 12:59, 22 October 2012