https://eln.excellgen.com/index.php?action=history&feed=atom&title=1430-pUltraBac-VVDP 2026-10-18T16:04:41Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1430-pUltraBac-VVDP&diff=6708&oldid=prev 2012-10-24T18:08:00Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1430-pUltraBac-VVDP&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1430-pUltraBac-VVDP <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-10-19---2012-10-24 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1430&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === subcloning ===<br /> <br /> [[Image:1430 clone 10-19-2012.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> {| width=&quot;482&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt; <br /> | band 1&lt;br&gt; <br /> | band 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | 1324,pCMV5-Fc(3C)-VVDP-WPRE-Orip &lt;br&gt; <br /> | 1246,pUltrabac&lt;br&gt;<br /> |-<br /> | enzymes&lt;br&gt; <br /> | colspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; BamH1+Not1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2012-10-22 <br /> <br /> Make miniprep and digest with BamH1+Not1 <br /> <br /> [[Image:1311+1430 digest detect 10-23-2012.JPG]]&lt;br&gt; <br /> <br /> clone 1,2,3,4 may be right <br /> <br /> <br /> <br /> Date: 2012-10-24&lt;br&gt; <br /> <br /> digest with Kpn1+BamH1<br /> <br /> [[Image:1430_digest_detect2_10-24-2012.JPG]]<br /> <br /> clone 1,2,3,4 may be right<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&amp;amp;lt;br&amp;amp;gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &amp;amp;lt;br&amp;amp;gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &amp;amp;lt;br&amp;amp;gt; <br /> <br /> {| cellspacing=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> {| cellspacing=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Category:Cloning]]</div>

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