https://eln.excellgen.com/index.php?action=history&feed=atom&title=1437_1458_1494_1497_1498_1505_1508 1437 1458 1494 1497 1498 1505 1508 - Revision history 2026-10-18T03:14:17Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1437_1458_1494_1497_1498_1505_1508&diff=8534&oldid=prev Paul.xu at 15:05, 12 March 2013 2013-03-12T15:05:13Z <p></p> <p><b>New page</b></p><div>Data:2012-12-29---2012-12-31 <br /> <br /> Exprimenter: yingying <br /> <br /> 3 ml sample each , 1437,1458,1494,1497,1505,1508&amp;nbsp; induce with 0.5mM IPTG at&amp;nbsp; 37 oC 0/N overnight&lt;br&gt; <br /> <br /> {| border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; style=&quot;width: 323px; height: 95px;&quot;<br /> |-<br /> | &#039;&#039;&#039;1437&#039;&#039;&#039; <br /> | &#039;&#039;&#039;pT7A3-RNaseA&#039;&#039;&#039;<br /> |-<br /> | &#039;&#039;&#039;1458&#039;&#039;&#039; <br /> | &#039;&#039;&#039;pT7A3-KOD&#039;&#039;&#039;<br /> |-<br /> | &#039;&#039;&#039;1494&#039;&#039;&#039; <br /> | &#039;&#039;&#039;pT7A3-UDG&#039;&#039;&#039;<br /> |-<br /> | 1497&lt;br&gt; <br /> | pT7A3-ToughTaq&lt;br&gt;<br /> |-<br /> | 1498&lt;br&gt; <br /> | pT7A3-TaqDNALigase&lt;br&gt;<br /> |-<br /> | 1505&lt;br&gt; <br /> | pT7A3-T7-Endo1&lt;br&gt;<br /> |-<br /> | 1508&lt;br&gt; <br /> | pT7A3-DNA-Pol-1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {| border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; width=&quot;200&quot;<br /> |-<br /> | &lt;br&gt; <br /> | 1 &lt;br&gt; <br /> | 2&lt;br&gt; <br /> | 3&lt;br&gt; <br /> | 4&lt;br&gt; <br /> | 5&lt;br&gt; <br /> | 6&lt;br&gt; <br /> | 7&lt;br&gt; <br /> | 8&lt;br&gt; <br /> | 9&lt;br&gt;<br /> |-<br /> | Clone ID&lt;br&gt; <br /> | 1437&lt;br&gt; <br /> | 1458&lt;br&gt; <br /> | 1494&lt;br&gt; <br /> | 1497&lt;br&gt; <br /> | 1498&lt;br&gt; <br /> | 1505&lt;br&gt; <br /> | 1508&lt;br&gt; <br /> | 1429&lt;br&gt; <br /> | 1429control &lt;br&gt;<br /> |-<br /> | competent cell&lt;br&gt; <br /> | colspan=&quot;9&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; Nico21 DE3 plysN&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:1437+1458+1494+1497+1498+1505+1508 protein screening 12-31-2012.jpg]]&lt;br&gt; <br /> <br /> &amp;nbsp;conclusion&amp;nbsp;: 1498 pT7A3-TaqDNALigase has low expression (lane5) 76KD&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; width=&quot;421&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> {| cellspacing=&quot;1&quot; width=&quot;421&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; width=&quot;421&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; width=&quot;413&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Category:Protein_Purification]]</div> Paul.xu