https://eln.excellgen.com/index.php?action=history&feed=atom&title=1438_pFUS_A-SMN2-L1 1438 pFUS A-SMN2-L1 - Revision history 2026-10-18T11:23:16Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1438_pFUS_A-SMN2-L1&diff=7144&oldid=prev Paul.xu at 20:48, 20 November 2012 2012-11-20T20:48:26Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1438 pFUS A-SMN2-L1&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1438 pFUS A-SMN2-L1<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-1--- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1438&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> Day2, pick white clones to PCR using primers pCR8_F1 and pCR8_R1&amp;nbsp;: <br /> <br /> [[Image:SMN2---1 TOP1 1-8 and TOP2 1-8.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> <br /> <br /> Day3&lt;br&gt; <br /> <br /> &amp;nbsp;Miniprep the&amp;nbsp; plasmids: Use enzymes AflII and XbaI to digest&lt;br&gt; <br /> <br /> [[Image:Smn2--smn1digest 11 02-2012.JPG]]&lt;br&gt; <br /> <br /> Top1-5,Top1-8,Bottom1-1, Bottom2-1,Bottom2-4,Bottom2-5 may be right.&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> Sequence TOP1-5,BOTTOM1-1,BOTTOM2-1 with primers pCR8_F1 and pCR8_R1.&lt;br&gt; <br /> <br /> TOP1-5,BOTTOM1-1,BOTTOM2-1 are right.&lt;br&gt; <br /> <br /> <br /> <br /> <br /> <br /> [[Category:Cloning]]</div> Paul.xu