Crm at 02:51, 19 November 2018

2018-11-19T02:51:21Z

New page

[[File:1002 1665 3274.JPG|RTENOTITLE]]

redo

[[File:142 143 145 digestion 10 23 2018.JPG|RTENOTITLE]]

[[File:142 143 undigested.JPG|RTENOTITLE]]

[[File:142 143 digestion.JPG|RTENOTITLE]]

[[File:142 143 145 digestion 10 23 2018.JPG|RTENOTITLE]]

[[File:1002 142 143 3274.JPG|RTENOTITLE]]

[[File:1002 141 1851 143.JPG|RTENOTITLE]]

[[File:142 143 digestion trouble shooting.JPG|RTENOTITLE]]

[[File:142 143 undigested1.JPG|RTENOTITLE]]

[[File:142 143 plasmid trouble shooting.JPG|RTENOTITLE]]

[[File:1002 142 143 3274 digestion.JPG|RTENOTITLE]]

[[File:142 143 undigested2.JPG|RTENOTITLE]]

[[File:142 143 plasmid trouble shooting1.JPG|RTENOTITLE]]

[[File:141 143 digestion 10 26 18.JPG|RTENOTITLE]]

10/27/2018 Redo PCR 

[[File:143 PCR.JPG|RTENOTITLE]]

[[File:143 3249 digestion.JPG|RTENOTITLE]]

need to check 143 clone #2.

[[File:143 PCR2.JPG|RTENOTITLE]][[File:143 PCR 3.JPG|RTENOTITLE]]

[[File:143 pminiT2 Age1 Xba1.JPG|RTENOTITLE]]

[[File:2369 Xba1 Nde1.JPG|RTENOTITLE]]

[[File:New143 pMiniT2 Nde1 Xba1.JPG|RTENOTITLE]]

sequencing: clone #2 no priming

[[File:143 PCR w DMSO.JPG|RTENOTITLE]]

[[File:143 2nd PCR.JPG|RTENOTITLE]]

[[File:143 3249 digestion2.JPG|RTENOTITLE]]

11/5/2018, use new primers 143-F2<br/>ATATATTCatg ACT AGT ggcaaaatcgaagaaggtaaac

143-MBP-R2         <br/>GAACCGGTGCCTCCACCGCCCTGGAAG TAAAGGTTTTC

[[File:143 MBP GST PCR2.JPG|RTENOTITLE]]

[[File:143 2nd PCR2.JPG|RTENOTITLE]]

[[File:3274 1851.JPG|RTENOTITLE]]

digest MBP-GST with BspH1-Nde1, clone into NcoI-Nde1 digested vector 1851

11/8/2018

[[File:143 PCR 11 8 2018.JPG|RTENOTITLE]]

[[File:143 PCR3.JPG]]
[[Category:Cloning]]

143 pT7A3-MBP-(Tev)-GST-(3C) - Revision history

Crm at 02:51, 19 November 2018