https://eln.excellgen.com/index.php?action=history&feed=atom&title=1441_pFus_B9-SMN2-R2 1441 pFus B9-SMN2-R2 - Revision history 2026-10-18T07:56:07Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1441_pFus_B9-SMN2-R2&diff=7150&oldid=prev Paul.xu: Created page with "== '''Cloning of 1441 pFus B9-SMN2-R2''' == '''Purpose:''' Cloning of 1441 pFus B9-SMN2-R2 '''Date: '''2012-1--- '''Notebook:0''' '''Page:''' '''Clone ID:1441''' '''Ex..." 2012-11-20T21:05:47Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 1441 pFus B9-SMN2-R2&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1441 pFus B9-SMN2-R2 &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-1--- &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone ID:1441&#039;&#039;&#039; &#039;&#039;&#039;Ex...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1441 pFus B9-SMN2-R2&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1441 pFus B9-SMN2-R2<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-1--- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1441&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> Day2, pick white clones to PCR using primers pCR8_F1 and pCR8_R1&amp;nbsp;: <br /> <br /> [[Image:SMN2---1 TOP1 1-8 and TOP2 1-8.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> <br /> <br /> Day3&lt;br&gt; <br /> <br /> &amp;nbsp;Miniprep the&amp;nbsp; plasmids: Use enzymes AflII and XbaI to digest&lt;br&gt; <br /> <br /> [[Image:Smn2--smn1digest 11 02-2012.JPG]]&lt;br&gt; <br /> <br /> Top1-5,Top1-8,Bottom1-1, Bottom2-1,Bottom2-4,Bottom2-5 may be right.&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> Sequence TOP1-5,BOTTOM1-1,BOTTOM2-1 with primers pCR8_F1 and pCR8_R1.&lt;br&gt; <br /> <br /> TOP1-5,BOTTOM1-1,BOTTOM2-1 are right.&lt;br&gt; <br /> <br /> <br /> <br /> <br /> <br /> [[Category:Cloning]]</div> Paul.xu