https://eln.excellgen.com/index.php?action=history&feed=atom&title=1444_pLVX-Kozak-FRT-TurboRFP-ODC-FRT-TurboGFP 1444 pLVX-Kozak-FRT-TurboRFP-ODC-FRT-TurboGFP - Revision history 2026-10-18T06:35:53Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1444_pLVX-Kozak-FRT-TurboRFP-ODC-FRT-TurboGFP&diff=7614&oldid=prev Paul.xu at 18:50, 21 December 2012 2012-12-21T18:50:04Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1444 pLVX-Kozak-FRT-TurboRFP-ODC-FRT-TurboGFP&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1444 pLVX-Kozak-FRT-TurboRFP-ODC-FRT-TurboGFP <br /> <br /> &#039;&#039;&#039;Date:Dec&#039;&#039;&#039;, 2012&lt;br&gt; <br /> <br /> &#039;&#039;&#039;Notebook:&amp;nbsp;&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID: &#039;&#039;&#039;1444 <br /> <br /> &#039;&#039;&#039;Experimenters: &#039;&#039;&#039; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Cloning ===<br /> <br /> PCR out TurboRFP-ODC from 1272 pLVX-loxP-TurboRFP-ODC-loxP-TurboGFP [[Image:549-1 549-2 1444 1478 1517 1480pcr.JPG]] &lt;br&gt; <br /> <br /> digestion 1427 pLVX-Kozak-loxP-TurboRFP-ODC-loxP-TurboGFP and PCR products with BamH1 + Xho1 <br /> <br /> [[Image:DIGEST FOR1478 1444 1528 1517.JPG]] <br /> <br /> === &lt;u&gt;&lt;/u&gt;Colony Screening&lt;br&gt; ===<br /> <br /> digestion with BamH1 + Xho1&lt;br&gt;<br /> <br /> [[Image:1444digest.JPG]]&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div> Paul.xu