https://eln.excellgen.com/index.php?action=history&feed=atom&title=1458_1654_1697 1458 1654 1697 - Revision history 2026-10-18T03:12:34Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1458_1654_1697&diff=8944&oldid=prev Paul.xu at 15:50, 22 April 2013 2013-04-22T15:50:05Z <p></p> <p><b>New page</b></p><div>Data:2013-4-11 <br /> <br /> Exprimenter: SHANYI&lt;br&gt; [[Image:1458 1654 1697after first purification.jpg]] <br /> <br /> {| width=&quot;200&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt; <br /> | colspan=&quot;2&quot; | 1&lt;br&gt; <br /> | 2&lt;br&gt; <br /> | colspan=&quot;2&quot; | 3&lt;br&gt; <br /> | colspan=&quot;2&quot; | 4&lt;br&gt; <br /> | 5&amp;nbsp;&amp;nbsp;&amp;nbsp; &lt;br&gt; <br /> | 6 &amp;nbsp;&amp;nbsp; &lt;br&gt; <br /> | &amp;nbsp;7 &amp;nbsp; &lt;br&gt; <br /> | &amp;nbsp;8 &amp;nbsp;&amp;nbsp; &lt;br&gt; <br /> | 10&lt;br&gt; <br /> | 11&lt;br&gt; <br /> | 12&lt;br&gt;<br /> |-<br /> | clone ID <br /> | colspan=&quot;5&quot; | 1458&lt;br&gt; <br /> | colspan=&quot;2&quot; | Marker&lt;br&gt;&lt;br&gt; <br /> | colspan=&quot;4&quot; | 1654&lt;br&gt; <br /> | colspan=&quot;4&quot; | 1697&lt;br&gt;<br /> |-<br /> | name&lt;br&gt; <br /> | colspan=&quot;5&quot; | pT7A3-KOD&lt;br&gt; <br /> | colspan=&quot;2&quot; | &lt;br&gt; <br /> | colspan=&quot;4&quot; | pT7A3-T3 DNA ligase&lt;br&gt; <br /> | colspan=&quot;4&quot; | pCold-GST-(3c)-wt3C&lt;br&gt;<br /> |-<br /> | host&lt;br&gt; <br /> | colspan=&quot;5&quot; | &lt;br&gt;Nico21DE3plysN&lt;br&gt; <br /> | colspan=&quot;2&quot; | &lt;br&gt;&lt;br&gt; <br /> | colspan=&quot;4&quot; | Nico21DE3plysN&lt;br&gt; <br /> | colspan=&quot;4&quot; | Nico21DE3plysN&lt;br&gt;<br /> |-<br /> | induction&lt;br&gt; <br /> | colspan=&quot;5&quot; | 0.2 mM IPTG 25oC o/n&lt;br&gt; <br /> | colspan=&quot;2&quot; | &lt;br&gt; <br /> | colspan=&quot;4&quot; | <br /> 0.2 mM IPTG 25oC o/n <br /> <br /> &lt;br&gt; <br /> <br /> | colspan=&quot;4&quot; | 0.2 mM IPTG 25oC o/n&lt;br&gt;<br /> |-<br /> | purification&lt;br&gt; <br /> | colspan=&quot;5&quot; | 10ml HisTrap HP&lt;br&gt; <br /> | colspan=&quot;2&quot; | &lt;br&gt; <br /> | colspan=&quot;4&quot; | 10ml HisTrap HP&lt;br&gt; <br /> | colspan=&quot;4&quot; | 10ml HisTrap HP&lt;br&gt;<br /> |-<br /> | MW&lt;br&gt; <br /> | colspan=&quot;5&quot; | 7.53 / 90022.42 &lt;br&gt; <br /> | colspan=&quot;2&quot; | &lt;br&gt; <br /> | colspan=&quot;4&quot; | 5.14 / 39350.70&lt;br&gt; <br /> | colspan=&quot;4&quot; | 21KD/8.47&lt;br&gt;<br /> |}<br /> <br /> [[Category:Protein_Purification]]</div> Paul.xu