https://eln.excellgen.com/index.php?action=history&feed=atom&title=1458_1654_1711_1712 1458 1654 1711 1712 - Revision history 2026-10-18T02:17:10Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1458_1654_1711_1712&diff=9059&oldid=prev Paul.xu at 19:11, 3 May 2013 2013-05-03T19:11:08Z <p></p> <p><b>New page</b></p><div>Data:2013-4-17 <br /> <br /> Exprimenter: SHANYI <br /> <br /> [[Image:1458after2ndNi 1654aftergel filtration 1711 1712small.jpg]]&lt;br&gt; <br /> <br /> {| border=&quot;1&quot; width=&quot;685&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt; <br /> | colspan=&quot;2&quot; | 1&lt;br&gt; <br /> | 2&lt;br&gt; <br /> | 3&lt;br&gt; <br /> | 4&lt;br&gt; <br /> | 5&lt;br&gt; <br /> | colspan=&quot;2&quot; | 6&lt;br&gt; <br /> | &amp;nbsp; 7 &amp;nbsp; &lt;br&gt; <br /> | &amp;nbsp;&amp;nbsp; 8&amp;nbsp; &lt;br&gt; <br /> | &amp;nbsp;&amp;nbsp;&amp;nbsp; 9 &lt;br&gt; <br /> | &amp;nbsp; 10&amp;nbsp; &lt;br&gt; <br /> | colspan=&quot;2&quot; | 11 &lt;br&gt; <br /> | 12&lt;br&gt;<br /> |-<br /> | clone ID <br /> | colspan=&quot;6&quot; | 1458&lt;br&gt; <br /> | colspan=&quot;2&quot; | Marker&lt;br&gt;&lt;br&gt; <br /> | colspan=&quot;4&quot; | 1654&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; &lt;br&gt; <br /> | colspan=&quot;2&quot; | <br /> 1711&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; &lt;br&gt; <br /> <br /> | colspan=&quot;2&quot; | <br /> &amp;nbsp;1712 &amp;nbsp; &lt;br&gt; <br /> <br /> |-<br /> | name&lt;br&gt; <br /> | colspan=&quot;6&quot; | pT7A3-KOD&lt;br&gt; <br /> | colspan=&quot;2&quot; | &lt;br&gt; <br /> | colspan=&quot;4&quot; | pT7A3-T3 DNA&amp;nbsp; ligase&lt;br&gt; <br /> | colspan=&quot;2&quot; | pET28b-hINFA2 &lt;br&gt; <br /> | colspan=&quot;2&quot; | pLambda-hINFA2&lt;br&gt;<br /> |-<br /> | host&lt;br&gt; <br /> | colspan=&quot;6&quot; | Nico21DE3plysN&lt;br&gt; <br /> | colspan=&quot;2&quot; | &lt;br&gt;&lt;br&gt; <br /> | colspan=&quot;4&quot; | Nico21DE3plysN&lt;br&gt; <br /> | colspan=&quot;2&quot; | BL21(DE3)pRare2pLysS&lt;br&gt; <br /> | colspan=&quot;2&quot; | BL21(DE3)pRare2pLysS&lt;br&gt;<br /> |-<br /> | induction&lt;br&gt; <br /> | colspan=&quot;6&quot; | 0.2 mM IPTG 25oC o/n&lt;br&gt; <br /> | colspan=&quot;2&quot; | &lt;br&gt; <br /> | colspan=&quot;4&quot; | <br /> 0.2 mM IPTG 25oC o/n&lt;br&gt; <br /> <br /> | colspan=&quot;2&quot; | 0.2 mM IPTG 25oC o/n&lt;br&gt; <br /> | colspan=&quot;2&quot; | 37 oc culture,OD=1.0, 42 oC induction 2h&lt;br&gt;<br /> |-<br /> | purification&lt;br&gt; <br /> | colspan=&quot;6&quot; | 10ml HisTrap HP(2nd time)&lt;br&gt; <br /> | colspan=&quot;2&quot; | &lt;br&gt; <br /> | colspan=&quot;4&quot; | Bio-Sil TSK 250&lt;br&gt; <br /> | colspan=&quot;2&quot; | Ni beads&lt;br&gt; <br /> | colspan=&quot;2&quot; | Ni beads&lt;br&gt;<br /> |-<br /> | MW&lt;br&gt; <br /> | colspan=&quot;6&quot; | 7.53 / 90022.42 &lt;br&gt; <br /> | colspan=&quot;2&quot; | &lt;br&gt; <br /> | colspan=&quot;4&quot; | 5.14 / 39350.70&lt;br&gt; <br /> | colspan=&quot;2&quot; | 21.6KD/5.95&lt;br&gt; <br /> | colspan=&quot;2&quot; | 21.6KD/5.95&lt;br&gt;<br /> |}<br /> <br /> [[Category:Protein_Purification]]</div> Paul.xu