https://eln.excellgen.com/index.php?action=history&feed=atom&title=1458_1779 1458 1779 - Revision history 2026-10-18T03:58:45Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1458_1779&diff=9847&oldid=prev Paul.xu: Created page with "== Protein Purification == 2013/7/25<br> SHANYI Image:1458_1779.jpg‎ <br> {| cellspacing="1" cellpadding="1" border="1" width="982" |- | Lane<br> | Name<br> | Prote..." 2013-07-26T16:41:05Z <p>Created page with &quot;== Protein Purification == 2013/7/25&lt;br&gt; SHANYI <a href="/index.php?title=File:1458_1779.jpg" title="File:1458 1779.jpg">Image:1458_1779.jpg‎ </a>&lt;br&gt; {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; width=&quot;982&quot; |- | Lane&lt;br&gt; | Name&lt;br&gt; | Prote...&quot;</p> <p><b>New page</b></p><div>== Protein Purification ==<br /> <br /> 2013/7/25&lt;br&gt; SHANYI <br /> <br /> [[Image:1458_1779.jpg‎ ]]&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; width=&quot;982&quot;<br /> |-<br /> | Lane&lt;br&gt; <br /> | Name&lt;br&gt; <br /> | Protein Name&lt;br&gt; <br /> | Clone ID&lt;br&gt; <br /> | Host&lt;br&gt; <br /> | Purification&lt;br&gt; <br /> | pI/Mw&lt;br&gt; <br /> | Expression &lt;br&gt;<br /> |-<br /> | 1&lt;br&gt; <br /> | rowspan=&quot;7&quot; | pT7A3-KOD&lt;br&gt;<br /> | rowspan=&quot;7&quot; | KOD&lt;br&gt;<br /> | rowspan=&quot;7&quot; | &amp;nbsp; 1458&lt;br&gt;<br /> | rowspan=&quot;7&quot; | Nico21DE3plysN&lt;br&gt;<br /> | rowspan=&quot;7&quot; | <br /> 10 ml Ni column&lt;br&gt;<br /> <br /> Bio-Sil TSK 250&lt;br&gt; &lt;br&gt;<br /> <br /> | rowspan=&quot;7&quot; | <br /> &lt;br&gt;<br /> <br /> 7.91 / 92215.77 &lt;br&gt; &lt;br&gt; &lt;br&gt;<br /> <br /> | rowspan=&quot;7&quot; | &lt;br&gt;&amp;nbsp; yes&lt;br&gt; &lt;br&gt; &lt;br&gt; &lt;br&gt;<br /> |-<br /> | 2&lt;br&gt;<br /> |-<br /> | 3&lt;br&gt;<br /> |-<br /> | 4&lt;br&gt;<br /> |-<br /> | 5&lt;br&gt;<br /> |-<br /> | 6&lt;br&gt;<br /> |-<br /> | 7&lt;br&gt;<br /> |-<br /> | 8&lt;br&gt; <br /> | PageRuler Plus Prestained protein ladder &lt;br&gt; <br /> | &lt;br&gt; <br /> | <br /> <br /> <br /> &lt;br&gt;<br /> <br /> | <br /> &amp;nbsp; <br /> <br /> &lt;br&gt;<br /> <br /> | <br /> After TEVdigestion <br /> <br /> elute from Histrap&lt;br&gt; <br /> <br /> | <br /> &lt;br&gt; <br /> <br /> | &lt;br&gt;<br /> |-<br /> | 9&lt;br&gt; <br /> | rowspan=&quot;4&quot; | <br /> &lt;br&gt;<br /> <br /> pTCold-MBP-(3C-TEV)-NRF2 &lt;br&gt; &lt;br&gt;<br /> <br /> | rowspan=&quot;4&quot; | NRF2 &lt;br&gt;<br /> | rowspan=&quot;4&quot; | <br /> &lt;br&gt;<br /> <br /> 1779 &lt;br&gt; &lt;br&gt;<br /> <br /> | rowspan=&quot;4&quot; | <br /> &amp;nbsp; &lt;br&gt;<br /> <br /> BL21DE3pRare2pLysS &lt;br&gt; &amp;nbsp; &lt;br&gt;<br /> <br /> | Flowthrough from&lt;br&gt;<br /> Histrap and&lt;br&gt;<br /> <br /> Amylose beads&lt;br&gt;<br /> <br /> | &amp;nbsp; 4.67 / 68345.09 &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 10&lt;br&gt; <br /> | Flowthrough from<br /> Histrap&lt;br&gt;<br /> <br /> | 4.67 / 68345.09 &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 11&lt;br&gt; <br /> | After TEVdigestion<br /> elute from Histrap&lt;br&gt;<br /> <br /> | &amp;nbsp; &lt;br&gt;&lt;br&gt; <br /> | &amp;nbsp; &lt;br&gt;<br /> |-<br /> | 12&lt;br&gt;<br /> | After TEVdigestion<br /> elute from Amylose beads&lt;br&gt;<br /> <br /> | &lt;br&gt;<br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Category:Protein_Purification]]</div> Paul.xu