https://eln.excellgen.com/index.php?action=history&feed=atom&title=145_pMiniT2-sfGFP
145 pMiniT2-sfGFP - Revision history
2026-10-18T12:33:28Z
Revision history for this page on the wiki
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https://eln.excellgen.com/index.php?title=145_pMiniT2-sfGFP&diff=19538&oldid=prev
Crm at 00:10, 31 October 2018
2018-10-31T00:10:16Z
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<p><b>New page</b></p><div>[[File:145 1.JPG|RTENOTITLE]]<br />
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[[File:145 2.JPG|RTENOTITLE]]<br />
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[[File:145 digestion.JPG|RTENOTITLE]]<br />
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[[File:145 digestion2.JPG|RTENOTITLE]]<br />
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[[File:145 digestion3.JPG|RTENOTITLE]]<br />
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sequencing: missing promoter sequence<br />
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Redo PCR with F2+ R1, then F1+ R1, and clone into pminiT2 using Bamh1+ PacI---> 145 fix<br />
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[[File:145-fix EcoRv PvuII.JPG|RTENOTITLE]]<br />
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[[File:3249 145Fix.JPG|RTENOTITLE]]<br />
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[[File:145fix digestion.JPG|RTENOTITLE]]<br />
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[[File:3283 145 3257.JPG|RTENOTITLE]]<br />
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[[File:145 EcoRV PvuII.JPG|RTENOTITLE]]<br />
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[[File:145Fix EcoRv PvuII.JPG]][[File:145Fix EcoRv PvuII.JPG]][[Category:Cloning]]</div>
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