https://eln.excellgen.com/index.php?action=history&feed=atom&title=1460_pAd5-LoxP-MCS-IRES-EGFP 1460 pAd5-LoxP-MCS-IRES-EGFP - Revision history 2026-10-18T20:33:00Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1460_pAd5-LoxP-MCS-IRES-EGFP&diff=7365&oldid=prev Paul.xu at 19:40, 6 December 2012 2012-12-06T19:40:24Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1460 pAd5-LoxP-MCS-IRES-EGFP&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1460 pAd5-LoxP-MCS-IRES-EGFP <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-11-21-- &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1460&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; SHANYI <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1396 1385 1454 533 1459digest.JPG]] digest 533 pLVX-Cre-IRES-EGFP with Xho1 +Mlu1--&amp;gt; IRES-GFP insert digest 1459 pAd5-LoxP-MCS with Sal1 +Mlu1--&amp;gt; vector <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2012-11- <br /> <br /> [[Image:1460digest.JPG]]&lt;br&gt; <br /> <br /> digestion with (EcoR1 + Mlu1) (note: Sal1 is gone) digestion&lt;br&gt; &lt;br&gt; <br /> <br /> = &lt;br&gt; =<br /> <br /> [[Category:Cloning]]</div> Paul.xu