Paul.xu at 20:50, 3 December 2012

2012-12-03T20:50:16Z

New page

== '''Cloning of 1463-pAD5-PR8-HAin''' ==

'''Purpose:''' Cloning of 1463-pAD5-PR8-HAin

'''Date: '''2012-11-20--2012-11-26

'''Notebook:0'''

'''Page:'''

'''Clone ID:1463'''

'''Experimenters:''' yingying

== '''Results''' ==

=== cloning ===

[[Image:1096+1463 PCR 11-20-2012.JPG]] 

PCR using 1462 pHW2000-PR8/HAin as template and

PR8-HAwt-BamH1-F PR8-HAwt-Not1-R

 

[[Image:240+1096+1463 clone 11-20-2012.JPG]] 

{| width="389" cellspacing="1" cellpadding="1" border="1"
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| lane
| 5
| 6
|-
| 
| PCR product 
| 280 pAd5-CMV-MCS 
|-
| enzymes 
| colspan="2" |              BamH1+Not1 
|}

 

=== Colony Screening ===

Date: 2012-11-25

[[Image:1463 2012 11 25.jpg]] 

plasmid yield is very low

 

2012-11-26

[[Image:1463 digest detect 11-26-2012.JPG]]

clone1,2 3,4,5,6 need to sequece

 

 

 

 

== '''Result intepretation/Summary''' ==

== ==

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== '''Conclusion''' ==

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== '''Cloning of 1463-pAD5-PR8-HAin''' ==

'''Purpose:''' Cloning of 1463-pAD5-PR8-HAin

'''Date: '''2012-11-29--2012-12-3

'''Notebook:0'''

'''Page:'''

'''Clone ID:1463'''

'''Experimenters:''' yingying

== '''Results''' ==

=== cloning ===

PCR using 1462 pHW2000-PR8/HAin (new) as template and

PR8-HAwt-BamH1-F PR8-HAwt-Not1-R

[[Image:1463_PCR_11-29-2012.JPG]]

 

[[Image:1463 clone 11-29-2012.JPG]] 

{| width="389" cellspacing="1" cellpadding="1" border="1"
|-
| lane
| 5
| 6
|-
| 
| PCR product 
| 280 pAd5-CMV-MCS 
|-
| enzymes 
| colspan="2" |              BamH1+Not1 
|}

 

=== Colony Screening ===

Date: 2012-12-3

[[Image:1463 diges detect 12-3-2012.JPG]] 

clone,2 ,4,5,6 need to sequece

 

 

 

 

== '''Result intepretation/Summary''' ==

== ==

*&amp;lt;br&amp;gt;

== '''Conclusion''' ==

&amp;lt;br&amp;gt;

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&amp;lt;br&amp;gt;

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&lt;br&gt;

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[[Category:Cloning]]

1463-pAD5-PR8-HAin - Revision history

Paul.xu at 20:50, 3 December 2012