https://eln.excellgen.com/index.php?action=history&feed=atom&title=1465_1510_1511_1514_1517 1465 1510 1511 1514 1517 - Revision history 2026-10-18T08:20:56Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1465_1510_1511_1514_1517&diff=7756&oldid=prev Paul.xu: Created page with "Data:2012-12-29---2012-12-31 Exprimenter: yingying 3 ml sample each , 1465,1510,1511,1517&nbsp; induce with 0.5mM IPTG at&nbsp; 37 oC 0/N overnight<br> {| cellspacing="1" c..." 2013-01-03T21:01:16Z <p>Created page with &quot;Data:2012-12-29---2012-12-31 Exprimenter: yingying 3 ml sample each , 1465,1510,1511,1517  induce with 0.5mM IPTG at  37 oC 0/N overnight&lt;br&gt; {| cellspacing=&quot;1&quot; c...&quot;</p> <p><b>New page</b></p><div>Data:2012-12-29---2012-12-31 <br /> <br /> Exprimenter: yingying <br /> <br /> 3 ml sample each , 1465,1510,1511,1517&amp;nbsp; induce with 0.5mM IPTG at&amp;nbsp; 37 oC 0/N overnight&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 323px; height: 95px;&quot;<br /> |-<br /> | &#039;&#039;&#039;1465&amp;nbsp;&#039;&#039;&#039; <br /> | &#039;&#039;&#039;pT7A3-MBP-3C&#039;&#039;&#039;<br /> |-<br /> | &#039;&#039;&#039;1510&#039;&#039;&#039; <br /> | &#039;&#039;&#039;pT7A3-MBP.Native-3C&#039;&#039;&#039;<br /> |-<br /> | &#039;&#039;&#039;1511&#039;&#039;&#039; <br /> | &#039;&#039;&#039;pT7A3-MBP.Synthetic-3C&#039;&#039;&#039;<br /> |-<br /> | 1514&lt;br&gt; <br /> | pT7A3-MBP-(3C)&lt;br&gt;<br /> |-<br /> | 1517&lt;br&gt; <br /> | pT7A3-MBP-(3C)-sfGFP&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {| width=&quot;509&quot; height=&quot;122&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> | 1&lt;br&gt;<br /> | 2&lt;br&gt;<br /> | 3&lt;br&gt;<br /> | 4&lt;br&gt;<br /> | 5&lt;br&gt;<br /> | 6&lt;br&gt;<br /> | 7&lt;br&gt;<br /> | 8&lt;br&gt;<br /> |-<br /> | Clone ID&lt;br&gt;<br /> | 1465&lt;br&gt;<br /> | 1511&lt;br&gt;<br /> | 1510&lt;br&gt;<br /> | 1511&lt;br&gt;<br /> | 1514&lt;br&gt;<br /> | 1517&lt;br&gt;<br /> | 1429&lt;br&gt;<br /> | 1429control &lt;br&gt;<br /> |-<br /> | competent cell&lt;br&gt;<br /> | colspan=&quot;2&quot; | BL21 Star DE3&lt;br&gt;<br /> | Rosetta2 DE3 plysS&lt;br&gt;<br /> | colspan=&quot;5&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; Nico21 DE3 plysN&lt;br&gt;<br /> |-<br /> | &amp;nbsp;&lt;br&gt;<br /> | &lt;br&gt;<br /> | &lt;br&gt;<br /> | &lt;br&gt;<br /> | &lt;br&gt;<br /> | &lt;br&gt;<br /> | &lt;br&gt;<br /> | &lt;br&gt;<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {| width=&quot;499&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |}<br /> <br /> [[Image:1465+1511+1510+1517 protein screening 12-31-2012.jpg]]&lt;br&gt; <br /> <br /> &amp;nbsp;conclusion&amp;nbsp;:1517 pT7A3-MBP-(3C)-sfGFP has&amp;nbsp; expression &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> {| width=&quot;421&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> {| width=&quot;421&quot; cellspacing=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {| width=&quot;421&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {| width=&quot;413&quot; cellspacing=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Category:Protein_Purification]]</div> Paul.xu