https://eln.excellgen.com/index.php?action=history&feed=atom&title=1466-pAD5-R4-Integrase 1466-pAD5-R4-Integrase - Revision history 2026-10-18T11:00:28Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1466-pAD5-R4-Integrase&diff=7884&oldid=prev Paul.xu at 17:54, 17 January 2013 2013-01-17T17:54:52Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1466-pAD5-R4-Integrase&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1466-pAD5-R4-Integrase <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-11-30---2012-12-19 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1466&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> gene synthesis&lt;br&gt;Frament 1 + Frament 2&lt;br&gt; <br /> <br /> [[Image:1466P12+1467 PCR 11-30-2012 .JPG]]&lt;br&gt; <br /> <br /> &amp;nbsp; <br /> <br /> join two fragments with PCR <br /> <br /> [[Image:1466 PCR 12-3-2012.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:1466+1470+1498 clone 12-17-2012.JPG]]&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2012-12-19 <br /> <br /> Make miniprep and digest with Kpn1+ Xho1&lt;br&gt; <br /> <br /> [[Image:1466 digest detect 12-19-2012.JPG]] <br /> <br /> clone 2,3,4,5,6 need to sequence <br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> c &amp;lt;br&amp;gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&amp;amp;amp;lt;br&amp;amp;amp;gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &amp;amp;amp;lt;br&amp;amp;amp;gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &amp;amp;amp;lt;br&amp;amp;amp;gt; <br /> <br /> {| cellspacing=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &amp;amp;lt;br&amp;amp;gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> == &#039;&#039;&#039;Cloning of 1466-pAD5-R4-Integrase&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1466-pAD5-R4-Integrase <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-1-10---2013-1-17<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1466&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> gene synthesis&lt;br&gt;Frament 1 + Frament 2&lt;br&gt; <br /> <br /> [[Image:1466 pcr1+2 1-8-2013.JPG]]&lt;br&gt; <br /> <br /> &amp;nbsp; <br /> <br /> join two fragments with PCR <br /> <br /> [[Image:1466 pcr 1-8-2013.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:1466+1543+1544+1584 clone 1-15-2013.JPG]]&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-1-17<br /> <br /> Make miniprep and digest with Kpn1+ Xho1&lt;br&gt; <br /> <br /> [[Image:1466_digest_detect_1-17-2013.JPG]]<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&amp;amp;amp;lt;br&amp;amp;amp;gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &amp;amp;amp;lt;br&amp;amp;amp;gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Category:Cloning]]</div> Paul.xu