https://eln.excellgen.com/index.php?action=history&feed=atom&title=1470-pCMV5-IgG-LC-Cripto-Fc%283c%29-WPRE-OriP 1470-pCMV5-IgG-LC-Cripto-Fc(3c)-WPRE-OriP - Revision history 2026-10-18T16:45:46Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1470-pCMV5-IgG-LC-Cripto-Fc(3c)-WPRE-OriP&diff=7664&oldid=prev Paul.xu at 18:26, 27 December 2012 2012-12-27T18:26:59Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1470-pCMV5-IgG-LC-Cripto-Fc(3c)-WPRE-OriP&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1470-pCMV5-IgG-LC-Cripto-Fc(3c)-WPRE-OriP <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-12-15---2012-12-16 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1470&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1470 PCR 12-6-2012.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:1466+1470+1498 clone 12-17-2012.JPG]]&lt;br&gt; <br /> <br /> lane 3:digest PCR product with Not1 + BglIII, &lt;br&gt; <br /> <br /> lane 4:digest 1395 pCMV5-hIgG-Lc-TagGFP-Fc(3c)-WPRE-OriP&amp;nbsp; with BamH1 + Not1&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> wrong <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2012- <br /> <br /> Make miniprep and digest with &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&amp;amp;amp;lt;br&amp;amp;amp;gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &amp;amp;amp;lt;br&amp;amp;amp;gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &amp;amp;amp;lt;br&amp;amp;amp;gt; <br /> <br /> {| cellspacing=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &amp;amp;lt;br&amp;amp;gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Cloning of 1470-pCMV5-IgG-LC-Cripto-Fc(3c)-WPRE-OriP&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1470-pCMV5-IgG-LC-Cripto-Fc(3c)-WPRE-OriP <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-12-20---2012-12-27<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1470&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> 2012-12-20&lt;br&gt; <br /> <br /> Template: 599 pLX304-cripto&lt;br&gt; <br /> <br /> 1 ul of 10 uM IgG-LC-BglII-BamH1-Nhe1-F1&lt;br&gt;1 ul of 1 uM IgG-LC-BglII-BamH1-Nhe1-F2&lt;br&gt; <br /> <br /> 1 ul of 10 uM Cripto-Not1-R&lt;br&gt; <br /> <br /> [[Image:1470 PCR 12-20-2012.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:1470 clone 12-20-2012.JPG]]&lt;br&gt; <br /> <br /> lane 1(low lane):digest PCR product with Not1 + BglIII, &lt;br&gt; <br /> <br /> lane 2:digest 1395 pCMV5-hIgG-Lc-TagGFP-Fc(3c)-WPRE-OriP&amp;nbsp; with BamH1 + Not1&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2012-12-27<br /> <br /> Make miniprep and digest with BamH1 + Kpn1&lt;br&gt; <br /> <br /> [[Image:1518+1470_digest_detect_12-27-2012.JPG]]<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&amp;amp;amp;lt;br&amp;amp;amp;gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &amp;amp;amp;lt;br&amp;amp;amp;gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &amp;amp;amp;lt;br&amp;amp;amp;gt; <br /> <br /> {| cellspacing=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &amp;amp;lt;br&amp;amp;gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Category:Cloning]]</div> Paul.xu