https://eln.excellgen.com/index.php?action=history&feed=atom&title=1472-1476 2026-10-18T11:15:52Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1472-1476&diff=7429&oldid=prev 2012-12-11T15:47:11Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1472-1476&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1472-1476 <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-11-26---2012-11-29 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1472-1476&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === subcloning ===<br /> <br /> [[Image:1424+1426+1472+1473+1474+1475+1476 clone 11-26-2012.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 281px; height: 66px;&quot;<br /> |-<br /> | &lt;br&gt; <br /> | band 1&lt;br&gt; <br /> | band 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | enzymes&lt;br&gt; <br /> | colspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2012-11-18 <br /> <br /> Make miniprep and digest with Nhe1 + Kpn1&lt;br&gt; <br /> <br /> [[Image:1472+1473+1474+1475+1476 digest detect 11-28-2012.JPG]]&lt;br&gt;<br /> <br /> 1472#3,4; 1473#1,2,3,4; 1475#1; 1476-#1; 1475#4 may be right, need to sequence <br /> <br /> 1472,1473,1474,1476 sequence result right, 1475 need to clone again&lt;br&gt;<br /> <br /> &lt;br&gt;<br /> <br /> 2012-12-4---2012-12-7&lt;br&gt;<br /> <br /> subclone : the same with above&lt;br&gt;<br /> <br /> [[Image:1466+1475_clone_12-4-2012.JPG]]&lt;br&gt;<br /> <br /> &lt;br&gt; <br /> <br /> mini prep and diges with Nhe1 + Kpn1&lt;br&gt;<br /> <br /> [[Image:1475_digest_detect_12-7-2012.JPG]]<br /> <br /> sequence result: 1475-6 right<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&amp;amp;lt;br&amp;amp;gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &amp;amp;lt;br&amp;amp;gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &amp;amp;lt;br&amp;amp;gt; <br /> <br /> {| cellspacing=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> {| cellspacing=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Category:Cloning]]</div>

Paul.xu