https://eln.excellgen.com/index.php?action=history&feed=atom&title=1477_pEC-WPRE 1477 pEC-WPRE - Revision history 2026-10-18T09:01:41Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1477_pEC-WPRE&diff=7287&oldid=prev Paul.xu at 18:37, 3 December 2012 2012-12-03T18:37:54Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1477 pEC-WPRE&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1477 pEC-WPRE <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-11-21-- &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1477&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; SHANYI <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1396 1385 1454 533 1459digest.JPG]] <br /> <br /> Digest 1385 pCMV5-TPA-GNE-(3c)-Fc-WPRE-oriP with Eag1 + Xba1--insert digest vector 1454 pEC with Not1 + Xba1. <br /> <br /> The band is not what we want.It should be 592bp.&lt;br&gt;<br /> <br /> &lt;br&gt;<br /> <br /> [[Image:1444pcr 1336 1454 1278pcr 1254digest.JPG]]&lt;br&gt;<br /> <br /> <br /> <br /> <br /> Digest 1336pCMV5-Fc(3C)-Dest-WPRE with Not1 + Xba1--insert digest vector 1454 pEC with Not1 + Xba1---vector&lt;br&gt;<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2012-12- <br /> <br /> <br /> [[Image:1147 1278 1477digest.JPG]] <br /> <br /> <br /> &lt;br&gt; &lt;br&gt; <br /> <br /> = &lt;br&gt; =<br /> <br /> [[Category:Cloning]]</div> Paul.xu