https://eln.excellgen.com/index.php?action=history&feed=atom&title=1483-pT7A3-T4_Ligase 1483-pT7A3-T4 Ligase - Revision history 2026-10-18T14:12:56Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1483-pT7A3-T4_Ligase&diff=7426&oldid=prev Paul.xu: Created page with "== '''Cloning of 1483-pT7A3-T4 Ligase''' == '''Purpose:''' Cloning of 1483-pT7A3-T4 Ligase '''Date: '''2012-12-3---2012-12-5 '''Notebook:0''' '''Page:''' '''Clone ID:148..." 2012-12-11T15:36:59Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 1483-pT7A3-T4 Ligase&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1483-pT7A3-T4 Ligase &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-12-3---2012-12-5 &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone ID:148...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1483-pT7A3-T4 Ligase&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1483-pT7A3-T4 Ligase<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-12-3---2012-12-5 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1483&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === subcloning ===<br /> <br /> [[Image:1467+1483+1500_clone_12-3-2012.JPG]]&lt;br&gt; <br /> <br /> {| width=&quot;374&quot; height=&quot;84&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> | lane 3&lt;br&gt;<br /> | lane 4&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | 240,pET28b-T4-Ligase&lt;br&gt;<br /> | 1279 pT7A3&lt;br&gt;<br /> |-<br /> | enzymes &lt;br&gt;<br /> | colspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp; NdeI + Xho1&lt;br&gt;<br /> |}<br /> <br /> <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |}<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2012-12-5 <br /> <br /> Make miniprep and digest withNdeI + Xho1<br /> <br /> [[Image:1467+1483+1500_digest_detect_12-05-2012.JPG]]<br /> <br /> clone 1,3,4,5 right<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&amp;amp;amp;lt;br&amp;amp;amp;gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &amp;amp;amp;lt;br&amp;amp;amp;gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |}<br /> <br /> &amp;amp;amp;lt;br&amp;amp;amp;gt;<br /> <br /> {| cellspacing=&quot;1&quot;<br /> |}<br /> <br /> &amp;amp;lt;br&amp;amp;gt;<br /> <br /> {|<br /> |}<br /> <br /> &amp;lt;br&amp;gt;<br /> <br /> {|<br /> |}<br /> <br /> <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Category:Cloning]]</div> Paul.xu