Paul.xu at 22:09, 3 January 2013

2013-01-03T22:09:26Z

New page

Data:2012-12-17---2012-12-20

Exprimenter: yingying

3 ml sample each , 1495,1496,1497  induce with 0.5mM IPTG at  37 oC 0/N overnight 

{| cellspacing="1" cellpadding="1" border="1" style="width: 323px; height: 95px;"
|-
| '''1495'''
| '''pT7A3-MMLV'''
|-
| '''1496'''
| '''pT7A3-DNase1'''
|-
| '''1497'''
| '''pT7A3-ToughTaq'''
|}

 

 

{| width="499" cellspacing="1" cellpadding="1" border="1"
|-
| lane
| 1
| 2
| 3
| 4
| 5
| 6
| 7
| 8
|-
| IPTG mM
| 0.5
| 0
| 0.5
| 0
| 0.5
| 0
| 0.5
| 0
|-
| rowspan="2" | clone ID 
| rowspan="2" colspan="2" | 1429 MBP 
| colspan="2" rowspan="2" | 1495 MMLV 
| colspan="2" rowspan="2" | 1496 Dnase1 
| colspan="2" rowspan="2" | 1497 ToughTaq 
|}

[[Image:1495+1496+1497 protein screen 12-21-2012.jpg]] 

 conclusion : ToughTaq has low expression (lane7) 70KD 

 

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[[Category:Protein_Purification]]

1495-1496-1497-screening - Revision history

Paul.xu at 22:09, 3 January 2013