https://eln.excellgen.com/index.php?action=history&feed=atom&title=1496-pT7A3-DNase1 1496-pT7A3-DNase1 - Revision history 2026-10-18T11:23:28Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1496-pT7A3-DNase1&diff=7424&oldid=prev Paul.xu: Created page with "== '''Cloning of 1496-pT7A3-DNase1''' == '''Purpose:''' Cloning of 1496-pT7A3-DNase1 '''Date: '''2012-12-5---2012-12-7 '''Notebook:0''' '''Page:''' '''Clone ID:1496''' ..." 2012-12-11T15:30:41Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 1496-pT7A3-DNase1&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1496-pT7A3-DNase1 &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-12-5---2012-12-7 &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone ID:1496&#039;&#039;&#039; ...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1496-pT7A3-DNase1&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1496-pT7A3-DNase1<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-12-5---2012-12-7<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1496&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === subcloning ===<br /> <br /> [[Image:1494+1495+1496+1497_clone_12-4-2012.JPG]]&lt;br&gt; <br /> <br /> {| width=&quot;366&quot; height=&quot;67&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> | lane 5&lt;br&gt;<br /> | lane 6&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | DNase1,202&lt;br&gt;<br /> | 1279 pT7A3&lt;br&gt;<br /> |-<br /> | enzymes &lt;br&gt;<br /> | colspan=&quot;2&quot; | Nco1 + Xho1&lt;br&gt;<br /> |}<br /> <br /> <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |}<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2012-12-7<br /> <br /> Make miniprep and digest with Nco1 + Xho1. <br /> <br /> [[Image:1496+1497_digest_detct_12-7-2012.JPG]]<br /> <br /> clone 1,2,3 right<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&amp;amp;amp;lt;br&amp;amp;amp;gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &amp;amp;amp;lt;br&amp;amp;amp;gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |}<br /> <br /> &amp;amp;amp;lt;br&amp;amp;amp;gt;<br /> <br /> {| cellspacing=&quot;1&quot;<br /> |}<br /> <br /> &amp;amp;lt;br&amp;amp;gt;<br /> <br /> {|<br /> |}<br /> <br /> &amp;lt;br&amp;gt;<br /> <br /> {|<br /> |}<br /> <br /> <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Category:Cloning]]</div> Paul.xu