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1497-pT7A3-ToughTaq - Revision history
2026-10-18T03:17:26Z
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Paul.xu at 18:43, 15 August 2013
2013-08-15T18:43:21Z
<p></p>
<p><b>New page</b></p><div>== '''Cloning of 1497-pT7A3-ToughTaq''' ==<br />
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'''Purpose:''' Cloning of 1497-pT7A3-ToughTaq <br />
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'''Date: '''2012-12-6----2012-12-11 <br />
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'''Notebook:0''' <br />
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'''Page:''' <br />
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'''Clone ID:1497''' <br />
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'''Experimenters:''' yingying <br />
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== '''Results''' ==<br />
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=== subcloning ===<br />
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[[Image:1494+1495+1496+1497 clone 12-4-2012.JPG]]<br> <br />
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{| cellspacing="1" cellpadding="1" border="1" width="368"<br />
|-<br />
| <br> <br />
| lane 7<br> <br />
| lane 8<br><br />
|-<br />
| <br> <br />
| 1151 pET30b-sso7d-ToughTaq<br> <br />
| 1279 pT7A3 <br><br />
|-<br />
| enzymes <br> <br />
| colspan="2" | &nbsp;&nbsp; Nde1 + Xho1<br><br />
|}<br />
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<br> <br />
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{| cellspacing="1" cellpadding="1" border="1"<br />
|-<br />
| <br><br />
|}<br />
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=== Colony Screening ===<br />
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Date: 2012-12-11 <br />
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Make miniprep and digest with Nde1 + Xho1<br> <br />
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[[Image:1496+1497 digest detct 12-7-2012.JPG]][[Image:1497 digest detect 12-11-2012.JPG]] <br />
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clone 5 right <br />
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== '''Result intepretation/Summary''' ==<br />
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*&amp;amp;lt;br&amp;amp;gt;<br />
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== '''Conclusion''' ==<br />
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&amp;amp;lt;br&amp;amp;gt; <br />
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<br><br />
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=== QC<br> ===<br />
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2013-8-15<br> <br />
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15ul elution in 300 mM Imidozale, 20 mM Tris-HCl pH 8.0, 600 mM NaCl <br> <br />
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PCR test <br> <br />
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[[Category:Cloning]]</div>
Paul.xu