https://eln.excellgen.com/index.php?action=history&feed=atom&title=1498_1797 1498 1797 - Revision history 2026-10-18T10:26:32Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1498_1797&diff=9949&oldid=prev Paul.xu at 16:30, 6 August 2013 2013-08-06T16:30:27Z <p></p> <p><b>New page</b></p><div>== Protein Purification ==<br /> <br /> 2013/8/2&lt;br&gt; SHANYI <br /> <br /> [[Image:1498 1797.jpg]]&lt;br&gt; <br /> <br /> 20ul sample from 1498 fraction16,19,24,33,37 and 1797fraction16,18,22,31,41.&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; width=&quot;982&quot;<br /> |-<br /> | Lane&lt;br&gt; <br /> | Name&lt;br&gt; <br /> | Protein Name&lt;br&gt; <br /> | Clone ID&lt;br&gt; <br /> | Host&lt;br&gt; <br /> | Purification&lt;br&gt; <br /> | pI/Mw&lt;br&gt; <br /> | Expression &lt;br&gt;<br /> |-<br /> | 1&lt;br&gt; <br /> | rowspan=&quot;7&quot; | pT7A3-TaqDNALigase&lt;br&gt; <br /> | rowspan=&quot;7&quot; | TaqDNALigase&lt;br&gt; <br /> | rowspan=&quot;7&quot; | &amp;nbsp; 1498&lt;br&gt; <br /> | rowspan=&quot;7&quot; | Nico21DE3plysN&lt;br&gt; <br /> | rowspan=&quot;7&quot; | <br /> 10 ml Ni column&lt;br&gt; <br /> <br /> Bio-Sil TSK 250&lt;br&gt; &lt;br&gt; <br /> <br /> | rowspan=&quot;7&quot; | <br /> 76KD&lt;br&gt; &lt;br&gt; <br /> <br /> | rowspan=&quot;7&quot; | &lt;br&gt;&amp;nbsp; yes&lt;br&gt; &lt;br&gt; &lt;br&gt; &lt;br&gt;<br /> |-<br /> | 2&lt;br&gt;<br /> |-<br /> | 3&lt;br&gt;<br /> |-<br /> | 4&lt;br&gt;<br /> |-<br /> | 5&lt;br&gt;<br /> |-<br /> | rowspan=&quot;2&quot; | 6 &lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 8&lt;br&gt; <br /> | rowspan=&quot;5&quot; | <br /> &lt;br&gt; <br /> <br /> Cold-6His-3C-MBP&lt;br&gt; &lt;br&gt; <br /> <br /> | rowspan=&quot;5&quot; | 3C-MBP&lt;br&gt; <br /> | rowspan=&quot;5&quot; | <br /> &lt;br&gt; <br /> <br /> 1797&lt;br&gt; &lt;br&gt; <br /> <br /> | rowspan=&quot;5&quot; | <br /> &amp;nbsp; &lt;br&gt; <br /> <br /> BL21(DE3)Star, pRare2-pLysS&lt;br&gt; &amp;nbsp; &lt;br&gt; <br /> <br /> | rowspan=&quot;5&quot; | 10 ml Ni column <br /> Bio-Sil TSK 250&lt;br&gt; <br /> <br /> | rowspan=&quot;5&quot; | &amp;nbsp; 6.01 / 62696.31 &lt;br&gt; &amp;nbsp; &lt;br&gt; &lt;br&gt; <br /> | rowspan=&quot;5&quot; | &lt;br&gt; &lt;br&gt; &amp;nbsp; &lt;br&gt; &lt;br&gt;<br /> |-<br /> | 9&lt;br&gt;<br /> |-<br /> | 10&lt;br&gt;<br /> |-<br /> | 11&lt;br&gt;<br /> |-<br /> | 12&lt;br&gt;<br /> |}<br /> <br /> [[Category:Protein_Purification]]</div> Paul.xu