https://eln.excellgen.com/index.php?action=history&feed=atom&title=1500_pT7A3-T3-ligase 2026-10-18T18:30:49Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1500_pT7A3-T3-ligase&diff=7425&oldid=prev 2012-12-11T15:33:49Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1500 pT7A3-T3-ligase&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1500 pT7A3-T3-ligase <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-12-3--2012-12-5<br /> <br /> &#039;&#039;&#039;Notebook: 0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1500&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === subcloning ===<br /> <br /> [[Image:1467+1483+1500_clone_12-3-2012.JPG]]&lt;br&gt;<br /> <br /> {| width=&quot;358&quot; height=&quot;100&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> | lane 5&lt;br&gt;<br /> | lane 6&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | 1096 pET28b-T3-Ligase&lt;br&gt;<br /> | 1279 pT7A3&lt;br&gt;<br /> |-<br /> | enzymes &lt;br&gt;<br /> | colspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; Nde1 +Xho1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2012-12-5<br /> <br /> mini prep and diges with Nde1 +Xho1&lt;br&gt;<br /> <br /> [[Image:1467+1483+1500_digest_detect_12-05-2012.JPG]]<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div>

Paul.xu