https://eln.excellgen.com/index.php?action=history&feed=atom&title=1504_pT7A3-T4DNA-Pol-Exo- 1504 pT7A3-T4DNA-Pol-Exo- - Revision history 2026-10-18T10:50:08Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1504_pT7A3-T4DNA-Pol-Exo-&diff=13297&oldid=prev Excellgen at 14:57, 26 September 2014 2014-09-26T14:57:25Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1504 pT7A3-T4DNA-Pol-Exo-&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of1504 pT7A3-T4DNA-Pol-Exo-&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-9---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1504&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> PCR using 1765 pET28b-T4DNAPol as template <br /> <br /> Fragment 1&lt;br&gt;T4DNAPol-1-1 CGACTAGCTAGCAAAGAATTTTATATCTCTATTGAAACAGTCGG&lt;br&gt;T4-p43D219A-R catgatatacggaacgGcaaacccctcaatattc <br /> <br /> Fragment 2&lt;br&gt;T4-p43D219A-F gaatattgaggggttt gCc gttccgtatatcatg&lt;br&gt;T4DNAPol-3-24 CGATCGGCGGCCGCTCAGCCAAACAGGAAGTCTAACGAAGCTTTTTCTTCATA&lt;br&gt; <br /> <br /> [[Image:2166ns-1319 2240 1504 2241pcr.JPG]][[Image:2328 1916 2199 2325 1504 2321 2345PCR.JPG]]&lt;br&gt; <br /> <br /> join two fragments by PCR <br /> <br /> [[Image:1504 2199 2321pcr.JPG]]&lt;br&gt; <br /> <br /> digest with NheI+NotI&lt;br&gt; <br /> <br /> [[Image:Digest for 1504 2321.JPG]]&lt;br&gt; <br /> <br /> [[Image:DIGEST FOR 2297 1504 1319 2240 2241 2246 2199.JPG]] <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014_8&lt;br&gt; <br /> <br /> Make miniprep, use&amp;nbsp; HindIII to find positive clone&lt;br&gt; <br /> <br /> [[Image:1319_1504_2241_2346digest.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Excellgen