https://eln.excellgen.com/index.php?action=history&feed=atom&title=1510_1511 1510 1511 - Revision history 2026-10-18T16:58:23Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1510_1511&diff=7936&oldid=prev Paul.xu at 14:37, 22 January 2013 2013-01-22T14:37:28Z <p></p> <p><b>New page</b></p><div>Data:2013-1-1---2013-1-4 <br /> <br /> Exprimenter: yingying <br /> <br /> 5 ml sample each , 1510,1511&amp;nbsp; induce with 0.2mM IPTG at&amp;nbsp; 37 oC 0/N overnight&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 323px; height: 95px;&quot;<br /> |-<br /> | &#039;&#039;&#039;1510&#039;&#039;&#039; <br /> | &#039;&#039;&#039;pT7A3-MBP.Native-3C&#039;&#039;&#039;<br /> |-<br /> | &#039;&#039;&#039;1511&#039;&#039;&#039; <br /> | &#039;&#039;&#039;pT7A3-MBP.Synthetic-3C&#039;&#039;&#039;<br /> |-<br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {| width=&quot;560&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt; <br /> | 1 &lt;br&gt; <br /> | 2&lt;br&gt; <br /> | 3&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | Clone ID&lt;br&gt; <br /> | 1510&lt;br&gt; <br /> | 1510&lt;br&gt; <br /> | 1511&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | competent cell&lt;br&gt; <br /> | &amp;nbsp; Rosetta2 plysS&lt;br&gt; <br /> | BL21 Star &lt;br&gt; <br /> | Nico21 DE3 plysN&lt;br&gt; <br /> | colspan=&quot;2&quot; | &lt;br&gt; <br /> | colspan=&quot;4&quot; | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:1510+1511 protein screen 1-4-2013.jpg]]&lt;br&gt; <br /> <br /> &amp;nbsp;conclusion&amp;nbsp;: only MBP express ,no 3C&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> 2013/1/7--2013/1/9&lt;br&gt; <br /> <br /> 0.1 mM IPTG &lt;br&gt; <br /> <br /> [[Image:1510+1511 protein screen 1-8-2013.jpg]]&lt;br&gt; <br /> <br /> {| width=&quot;535&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt; <br /> | 1&lt;br&gt; <br /> | 2&lt;br&gt; <br /> | 3&lt;br&gt; <br /> | 4&lt;br&gt; <br /> | 5&lt;br&gt; <br /> | 6&lt;br&gt;<br /> |-<br /> | Clone ID&lt;br&gt; <br /> | 1510&lt;br&gt; <br /> | 1510&lt;br&gt; <br /> | 1511&lt;br&gt; <br /> | 1510&lt;br&gt; <br /> | 1510&lt;br&gt; <br /> | 1511&lt;br&gt;<br /> |-<br /> | Competent cell &lt;br&gt; <br /> | BL21 Star&lt;br&gt; <br /> | Rosetta2 plysS &lt;br&gt; <br /> | Nico21DE3 plysN&lt;br&gt; <br /> | BL21 Star&lt;br&gt; <br /> | Rosetta2 plysS &lt;br&gt; <br /> | Nico21DE3 plysN&lt;br&gt;<br /> |-<br /> | Temp&lt;br&gt; <br /> | colspan=&quot;3&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp; 4 C overnight <br /> | colspan=&quot;3&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; 18.5 C overnight<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> 2013-1-13---2013-1-15 <br /> <br /> [[Image:1510+1511 protein sreening 1-15-2013.JPG]] <br /> <br /> {| width=&quot;395&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | <br /> | 1 <br /> | 2 <br /> | 3 <br /> | 4 <br /> | 5 <br /> | 6<br /> |-<br /> | clone ID <br /> | colspan=&quot;4&quot; | 1510 <br /> | 1510 <br /> | 1511<br /> |-<br /> | IPTG mM <br /> | 0.01 <br /> | 0.02 <br /> | 0.05 <br /> | 0.1 <br /> | 0.1 <br /> | 0.1<br /> |-<br /> | competent cell <br /> | colspan=&quot;4&quot; | BL21 Star <br /> | Rosetta2 DE3 plysS <br /> | Nico21DE3 plysN<br /> |-<br /> | Temp <br /> | colspan=&quot;6&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; 25C overnight<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {| width=&quot;421&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> {| width=&quot;421&quot; cellspacing=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {| width=&quot;421&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {| width=&quot;413&quot; cellspacing=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Category:Protein_Purification]]</div> Paul.xu