https://eln.excellgen.com/index.php?action=history&feed=atom&title=1519-pT7A3-T3-DNA-Ligase 2026-10-18T16:48:32Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1519-pT7A3-T3-DNA-Ligase&diff=11322&oldid=prev 2014-02-25T19:57:06Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1519-pT7A3-T3-DNA-Ligase&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1519-pT7A3-T3-DNA-Ligase <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-1-21------2013-1-24 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1519&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1519 clone 1-22-2012.JPG]]&lt;br&gt; <br /> <br /> subclone from 1518 pET28b-T3-DNA-Ligase to&amp;nbsp; 1279 pT7A3 using Nde1 + Xho1&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-1-24 <br /> <br /> Make miniprep and digest with&amp;nbsp; Nde1 + Xho1 <br /> <br /> [[Image:1519 digest detect 1-24-2013.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div>

Paul.xu