https://eln.excellgen.com/index.php?action=history&feed=atom&title=1524-pCDNA3.1-NLS-iCre 1524-pCDNA3.1-NLS-iCre - Revision history 2026-10-18T11:52:23Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1524-pCDNA3.1-NLS-iCre&diff=11504&oldid=prev Paul.xu at 18:39, 19 March 2014 2014-03-19T18:39:55Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1524-pCDNA3.1-NLS-iCre&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1524-pCDNA3.1-NLS-iCre <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-3-13---2014-3-14 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1524&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1524_2111clone_3-12-2014.JPG]]&lt;br&gt; <br /> <br /> {| width=&quot;225&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt; <br /> | 1,3&lt;br&gt; <br /> | 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | 664 pLVX-CMV-iCre-IRES-EGFP&lt;br&gt; <br /> | 1226 pCDNA3.1&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | colspan=&quot;2&quot; | BamH1 + Xho1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-3-14<br /> <br /> Make miniprep and digest with BamH1 + Xho1&lt;br&gt;<br /> <br /> [[Image:1524_2111digest_3-13-2014.JPG]]&lt;br&gt;<br /> <br /> &lt;br&gt;<br /> <br /> &lt;br&gt;<br /> <br /> [[Image:1520ddigst 3-10-2014.JPG]] <br /> <br /> {| width=&quot;246&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt; <br /> | 1&lt;br&gt; <br /> | 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | BamH1&lt;br&gt; <br /> | Xba1&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | colspan=&quot;2&quot; | 1520, pDIRE&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu