https://eln.excellgen.com/index.php?action=history&feed=atom&title=1526-pCDNA3.1-NLS-FLPo 1526-pCDNA3.1-NLS-FLPo - Revision history 2026-10-18T16:58:43Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1526-pCDNA3.1-NLS-FLPo&diff=7663&oldid=prev Paul.xu at 18:11, 27 December 2012 2012-12-27T18:11:29Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1526-pCDNA3.1-NLS-FLPo&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1483-pT7A3-T4 Ligase <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-12-20--2012-12-27<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1526&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1526 pcr 12-21-2012.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:1525+1526 clone 12-21-2012.JPG]]&lt;br&gt; <br /> <br /> &amp;nbsp;PCR product with BamH1 + Xho1 and clone into BamH1 + Xho1 digested 1226 pCDNA3.1+&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> {| width=&quot;374&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2012-12-27<br /> <br /> Make miniprep and digest with BamH1 + Xho1<br /> <br /> [[Image:1525+1526_digest_detect_12-27-2012.JPG]]<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&amp;amp;amp;amp;lt;br&amp;amp;amp;amp;gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &amp;amp;amp;amp;lt;br&amp;amp;amp;amp;gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Category:Cloning]]</div> Paul.xu