https://eln.excellgen.com/index.php?action=history&feed=atom&title=1528_pET28b.Amp.HC 2026-10-18T15:06:04Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1528_pET28b.Amp.HC&diff=8080&oldid=prev 2013-01-31T17:15:17Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1528 pET28b.Amp.HC&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1528 pET28b.Amp.HC <br /> <br /> &#039;&#039;&#039;Date:Dec&#039;&#039;&#039;, 2012&lt;br&gt; <br /> <br /> &#039;&#039;&#039;Notebook:&amp;nbsp;&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID: &#039;&#039;&#039;1528 <br /> <br /> &#039;&#039;&#039;Experimenters: &#039;&#039;&#039; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Cloning ===<br /> <br /> [[Image:1240 1429 pRestA v12digest for 1510 1528.JPG]]&lt;br&gt; <br /> <br /> digest pET28b with DraIII + Sap1, --&amp;gt; vector digest pRestA with DraIII+ Sap1--&amp;gt; 2171 bp fragment. <br /> <br /> === &lt;u&gt;&lt;/u&gt;Colony Screening&lt;br&gt; ===<br /> <br /> digest with BamHI and BsaI <br /> <br /> [[Image:1528digest.JPG]] <br /> <br /> Use sample1 .&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> 1528-1 digest with ScaI + SapI&lt;br&gt; <br /> <br /> puc19 digest with ScaI + SapI+NdeI&lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div>

Paul.xu