https://eln.excellgen.com/index.php?action=history&feed=atom&title=1532_pT7A3-T4-DNA-helicase 1532 pT7A3-T4-DNA-helicase - Revision history 2026-10-18T14:44:58Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1532_pT7A3-T4-DNA-helicase&diff=8117&oldid=prev Paul.xu at 19:16, 4 February 2013 2013-02-04T19:16:23Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1532 pT7A3-T4-DNA-helicase&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1532 pT7A3-T4-DNA-helicase <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;Jan , 2013&lt;br&gt; <br /> <br /> &#039;&#039;&#039;Notebook:&amp;nbsp;&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID: 1532&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters: &#039;&#039;&#039;Shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Cloning ===<br /> <br /> PCR&lt;br&gt; <br /> <br /> [[Image:1532 1538-2 1560 1561-1562 1563pcr.JPG]]&lt;br&gt; <br /> <br /> Nde1 + Xho1&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony screen ===<br /> <br /> [[Image:1529 1532 1538digest.JPG]] <br /> <br /> SEND 1532-4 TO SEQUENCE, IT IS T3 DNA ligase, SO CREATE A NEW NUMBER AS 1654.&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> PCR&lt;br&gt; <br /> <br /> [[Image:1532 1561 1480 1546 1547pcr 1 25 2013.JPG]]&lt;br&gt; <br /> <br /> digest with Nde1 + Xho1&lt;br&gt; <br /> <br /> [[Image:Digest for 1532 1562 1563.JPG]]&lt;br&gt; <br /> <br /> === Colony screen ===<br /> <br /> digest with Nde1 + Xho1&lt;br&gt; <br /> <br /> [[Image:1532DIGEST.JPG‎]]<br /> <br /> [[Category:Cloning]]</div> Paul.xu