https://eln.excellgen.com/index.php?action=history&feed=atom&title=1538_pT7A3-MBP-%283C%29-T4PNK-Pase- 1538 pT7A3-MBP-(3C)-T4PNK-Pase- - Revision history 2026-10-18T11:28:32Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1538_pT7A3-MBP-(3C)-T4PNK-Pase-&diff=8012&oldid=prev Paul.xu at 21:38, 28 January 2013 2013-01-28T21:38:22Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1538 pT7A3-MBP-(3C)-T4PNK-Pase-&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1538 pT7A3-MBP-(3C)-T4PNK-Pase- <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;Jan , 2013&lt;br&gt; <br /> <br /> &#039;&#039;&#039;Notebook:&amp;nbsp;&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID: 1538&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters: &#039;&#039;&#039;Shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Cloning ===<br /> <br /> PCR1&lt;br&gt; <br /> <br /> [[Image:1044 1045 1538-1pcr1 15 2013.JPG]] <br /> <br /> [[Image:1538-2pcr.JPG]]&lt;br&gt; <br /> <br /> 2 <br /> <br /> [[Image:1538 1 2 3pcr.JPG]] <br /> <br /> DIGEST <br /> <br /> [[Image:1538PCR 1550DIGEST.JPG]] <br /> <br /> &amp;nbsp;PCR<br /> <br /> 45oc 30s, 65oc 2min, 5cycle, 50oc 30s, 65oc 2min, 24cycle<br /> <br /> [[Image:1538PCR_1_23_2013.JPG‎ ]]<br /> <br /> [[Image:DIGEST_FOR_1044_1538_1_23_2013.JPG]]<br /> <br /> === Colony screen ===<br /> <br /> [[Image:1538digest_1_28_2013.JPG‎ ]]<br /> <br /> digest with NdeI and XhoI<br /> <br /> [[Category:Cloning]]</div> Paul.xu