https://eln.excellgen.com/index.php?action=history&feed=atom&title=153_pLVX-Plus 153 pLVX-Plus - Revision history 2026-10-18T10:30:08Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=153_pLVX-Plus&diff=5607&oldid=prev Jennifer.truong: Created page with "== '''Subcloning of 153 pLVX-Plus''' == '''Purpose:''' Subcloning of 153 pLVX-Plus '''Date: '''August 15, 2012<br> '''Notebook:&nbsp;''' '''Page:''' '''Clone ID: '''153 ..." 2012-08-15T18:52:26Z <p>Created page with &quot;== &#039;&#039;&#039;Subcloning of 153 pLVX-Plus&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Subcloning of 153 pLVX-Plus &#039;&#039;&#039;Date: &#039;&#039;&#039;August 15, 2012&lt;br&gt; &#039;&#039;&#039;Notebook: &#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone ID: &#039;&#039;&#039;153 ...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Subcloning of 153 pLVX-Plus&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Subcloning of 153 pLVX-Plus<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;August 15, 2012&lt;br&gt; <br /> <br /> &#039;&#039;&#039;Notebook:&amp;nbsp;&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID: &#039;&#039;&#039;153 <br /> <br /> &#039;&#039;&#039;Experimenters: &#039;&#039;&#039;Paul Xu and Jennifer Truong <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Cloning&lt;br&gt; ===<br /> <br /> cut vector 90 pLVX-puro-HA-AKR1C4 with Xho1+BamH1, purify vector&lt;br&gt;mix equal vol of pLVXPuro-F and pLVXPuro-R, heat at 95 oC for 5 min&lt;br&gt;Slowly cool to RT, mix with vector and ligate.&lt;br&gt;pLVXPuro-F&lt;br&gt;TCGAGGGCCGCTGCGGCCTTCGAATTCTGCAGTCGACGGTAGGCCAAACAGGCCG&lt;br&gt; CCCGGCGACGCCGGAAGCTTAAGACGTCAGCTGCCATCCGGTTTGTCCGGCCTAG<br /> <br /> pLVXPuro-R&lt;br&gt;GATCCGGCCTGTTTGGCCTACCGTCGACTGCAGAATTCGAAGGCCGCAGCGGCCC&lt;br&gt;<br /> <br /> === &lt;u&gt;&lt;/u&gt;Colony Screening&lt;br&gt; ===<br /> <br /> Date:&amp;nbsp;August 15, 2012&lt;br&gt; <br /> <br /> Digested MiniPrep with RE Nde1 + EcoR1 to cut out CMV promoter&lt;br&gt;<br /> <br /> [[Image:153_0815_Screening.JPG]]<br /> <br /> All samples positive for insert, used sample 1 for subcloning (all plasmids are inside Jennifer&#039;s box)<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div> Jennifer.truong