https://eln.excellgen.com/index.php?action=history&feed=atom&title=1540-pET30b-Native-ToughTaq 2026-10-18T03:17:23Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1540-pET30b-Native-ToughTaq&diff=8296&oldid=prev 2013-02-20T20:15:42Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1540-pET28b-Native-ToughTaq&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1540-pET28b-Native-ToughTaq <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-1-18---2013-1-31 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1540&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1540pcr1+2+1543+1544+1554 pcr 1-2-2013.JPG]] <br /> <br /> [[Image:1540 pcr 1-18-2013.JPG]]&lt;br&gt; <br /> <br /> [[Image:1540 clone 1-18-2013.JPG]] <br /> <br /> Clone into Kpn1 + Sal1 digested pET28b&lt;br&gt; <br /> <br /> &#039;&#039;&#039;wrong vector&#039;&#039;&#039;&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-1-31 <br /> <br /> Make miniprep and digest with&amp;nbsp; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&amp;amp;amp;lt;br&amp;amp;amp;gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &amp;amp;amp;lt;br&amp;amp;amp;gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &amp;amp;amp;lt;br&amp;amp;amp;gt; <br /> <br /> {| cellspacing=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &amp;amp;lt;br&amp;amp;gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Cloning of 1540-pET28b-Native-ToughTaq&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1540-pET28b-Native-ToughTaq <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-2-1--- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1540&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1540pcr1+2+1543+1544+1554 pcr 1-2-2013.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:1540+1629 clone 2-4-2013.JPG]]&lt;br&gt; <br /> <br /> &amp;nbsp;Clone into&amp;nbsp; Kpn1 + Sal1 digested pET28b&lt;br&gt;&lt;br&gt;&#039;&#039;&#039;wrong vector&#039;&#039;&#039; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Cloning of 1540-pET30b-Native-ToughTaq&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1540-pET28b-Native-ToughTaq <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-2-14---2013-2-20 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1540&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1540pcr1+2+1543+1544+1554 pcr 1-2-2013.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:554+560+1540 clone 2-14-2013.JPG]]&lt;br&gt; <br /> <br /> Clone into Kpn1 + Sal1 digested pET30b&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-2-19 <br /> <br /> Make miniprep and digest with&amp;nbsp; Kpn1 + Sal1 <br /> <br /> [[Image:1540+1584 digest detct 2-19-2013.JPG]][[Image:554+1540 digest detect 2-20-2013.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&amp;amp;amp;lt;br&amp;amp;amp;gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Category:Cloning]]</div>

Paul.xu