https://eln.excellgen.com/index.php?action=history&feed=atom&title=1542_1544_1545_1627_1679_1680 1542 1544 1545 1627 1679 1680 - Revision history 2026-10-18T02:18:11Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1542_1544_1545_1627_1679_1680&diff=8744&oldid=prev Paul.xu: moved 1542 1544 1545 1679 1680 to 1542 1544 1545 1627 1679 1680 2013-04-01T15:18:43Z <p>moved <a href="/index.php?title=1542_1544_1545_1679_1680" class="mw-redirect" title="1542 1544 1545 1679 1680">1542 1544 1545 1679 1680</a> to <a href="/index.php?title=1542_1544_1545_1627_1679_1680" title="1542 1544 1545 1627 1679 1680">1542 1544 1545 1627 1679 1680</a></p> <p><b>New page</b></p><div>2013/3/28 <br /> <br /> [[Image:1542 1544 1545 1627 1679 1680protein screen 3-28-2013.JPG]] <br /> <br /> {| border=&quot;1&quot; width=&quot;693&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt; <br /> | name&lt;br&gt; <br /> | clone ID&lt;br&gt; <br /> | host&lt;br&gt; <br /> | induction&lt;br&gt; <br /> | purification&lt;br&gt; <br /> | MW&lt;br&gt;<br /> |-<br /> | 1&lt;br&gt; <br /> | pT7A3-sso7d-Native-ToughTaq&lt;br&gt; <br /> | 1542&lt;br&gt; <br /> | 4 ml, <br /> BL21(DE3)Star, pRare2-pLysS &lt;br&gt; <br /> <br /> | 0.2 mM IPTG 25 oC o/n&lt;br&gt; <br /> | Ni beads&lt;br&gt; <br /> | 62KD&lt;br&gt;<br /> |-<br /> | 2&lt;br&gt; <br /> | pT7A3-VVCap-L&lt;br&gt; <br /> | 1544&lt;br&gt; <br /> | 4 ml, <br /> BL21(DE3)Star, pRare2-pLysS &lt;br&gt; <br /> <br /> | 0.2 mM IPTG 25 oC o/n&lt;br&gt; <br /> | Ni beads&lt;br&gt; <br /> | 34KD&lt;br&gt;<br /> |-<br /> | 3&lt;br&gt; <br /> | pT7A3-VVCap&lt;br&gt; <br /> | 1545&lt;br&gt; <br /> | 4 ml, <br /> BL21(DE3)Star, pRare2-pLysS &lt;br&gt; <br /> <br /> | 0.2 mM IPTG 25 oC o/n&lt;br&gt; <br /> | Ni beads&lt;br&gt; <br /> | 34KD&lt;br&gt;<br /> |-<br /> | 4&lt;br&gt; <br /> | pT7A3-SOD&lt;br&gt; <br /> | 1627&lt;br&gt; <br /> | 4 ml, <br /> BL21(DE3)Star, pRare2-pLysS &lt;br&gt; <br /> <br /> | 0.2 mM IPTG 25 oC o/n&lt;br&gt; <br /> | Ni beads&lt;br&gt; <br /> | 16KD&lt;br&gt;<br /> |-<br /> | 5&lt;br&gt; <br /> | pCold-MBP-SM2&lt;br&gt; <br /> | 1679&lt;br&gt; <br /> | 4 ml,NiCO21(DE3)pLysN,&lt;br&gt; <br /> | 0.2 mM IPTG 25 oC o/n&lt;br&gt; <br /> | Ni beads&lt;br&gt; <br /> | 74KD&lt;br&gt;<br /> |-<br /> | 6&lt;br&gt; <br /> | pCold-GST-wtSM2&lt;br&gt; <br /> | 1680&lt;br&gt; <br /> | 4 ml,NiCO21(DE3)pLysN,&lt;br&gt; <br /> | 0.2 mM IPTG 25 oC o/n&lt;br&gt; <br /> | get lysis supernatant to run gel&lt;br&gt; <br /> | 94KD&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;&lt;br&gt; <br /> <br /> [[Category:Protein_Purification]]</div> Paul.xu