https://eln.excellgen.com/index.php?action=history&feed=atom&title=1547_PiggyBacHR-SMN1-3HA 2026-10-18T15:36:14Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1547_PiggyBacHR-SMN1-3HA&diff=8087&oldid=prev 2013-01-31T19:02:04Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1547 PiggyBacHR-SMN1-3HA&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1547 PiggyBacHR-SMN1-3HA <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-1-28---2013-1-30 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1547&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; Shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039;&lt;br&gt; ==<br /> <br /> === PCR ===<br /> <br /> PCR from 293 genomic DNA&lt;br&gt;digest with Sap1 + Mfe1, clone into EcoR1 + Sap1 digested 1507 PiggyBacHR <br /> <br /> EcoR1 in the vector is gone, but there are two internal EcoR1 sites <br /> <br /> &lt;br&gt; [[Image:1532 1561 1480 1546 1547pcr 1 25 2013.JPG]]&lt;br&gt; <br /> <br /> [[Image:DIGEST FOR1547.JPG]]&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 281px; height: 66px;&quot;<br /> |-<br /> | &lt;br&gt;<br /> | band 2&lt;br&gt; <br /> | band 3,4,5&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | 1507&lt;br&gt; <br /> | 1547PCR&lt;br&gt;<br /> |-<br /> | enzymes&lt;br&gt; <br /> | colspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; SapI + Mfe1/EcoR1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013- <br /> <br /> Make miniprep and digest withEcoR1=&amp;gt;215bp<br /> <br /> [[Image:1547digest.JPG]]<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&amp;amp;lt;br&amp;amp;gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &amp;amp;lt;br&amp;amp;gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &amp;amp;lt;br&amp;amp;gt; <br /> <br /> {| cellspacing=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> {| cellspacing=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Category:Cloning]]</div>

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