https://eln.excellgen.com/index.php?action=history&feed=atom&title=1548_PiggyBacHR-SMN1-5-3HA 2026-10-18T13:22:39Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1548_PiggyBacHR-SMN1-5-3HA&diff=8709&oldid=prev 2013-03-29T13:25:20Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1548 PiggyBacHR-SMN1-5-3HA&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1548 PiggyBacHR-SMN1-5-3HA <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-2-11--- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1548&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; Shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039;&lt;br&gt; ==<br /> <br /> === PCR ===<br /> <br /> PCR from 293 genomic DNA&lt;br&gt; <br /> <br /> [[Image:1546PCR 3rd TIME.JPG]]&lt;br&gt;&lt;br&gt; <br /> <br /> Method: use 1547-2 as vector&lt;br&gt; <br /> <br /> digest and ligate together 10x (37 ̊C for 5min + 16 ̊C for 10min) + 50 ̊C for 5min + 80 ̊C for 5min &lt;br&gt;&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> pcr <br /> <br /> [[Image:1548CLONY SCREEN.JPG]]&lt;br&gt; &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> use plasmid 1234567as template <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039;&lt;br&gt; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div>

Paul.xu