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1550 pT7A3-MBP-(3C)-T4-DNA-Ligase - Revision history
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Paul.xu at 16:16, 31 December 2012
2012-12-31T16:16:33Z
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<p><b>New page</b></p><div>== '''Cloning of 1550 pT7A3-MBP-(3C)-T4-DNA-Ligase''' ==<br />
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'''Purpose:''' Cloning of 1550 pT7A3-MBP-(3C)-T4-DNA-Ligase<br />
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'''Date: '''December 27, 2012<br> <br />
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'''Notebook:&nbsp;''' <br />
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'''Page:''' <br />
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'''Clone ID: '''1550 <br />
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'''Experimenters: '''Jennifer Truong <br />
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== '''Results''' ==<br />
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=== Cloning ===<br />
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Date:&nbsp;December 26, 2012<br />
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Subclone T4 DNA ligase from 1483 pT7A3-T4_DNA_Ligase using Nde1 + Xho1<br><br />
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Vector Name 1514 pT7A3-MBP-(3C)<br><br />
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[[Image:1550_Subcloning.JPG]]<br />
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Ligate lane 3 (insert 1483) together with lane 4 vector (1514)<br />
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Transform<br />
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=== Colony Screening ===<br />
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Date:&nbsp;December 31, 2012<br />
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Picked colonies from plate, grew up 6 samples, Miniprepped<br />
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Digested plasmids with Xho1 and Nde1<br />
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[[Image:1123_1550_Screening.JPG]]<br />
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See lanes 7-12, samples 2-6 look like they have inserts <br />
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Submitted lane 12 (sample 6) for sequencing<br />
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== '''Result intepretation/Summary''' ==<br />
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== '''Conclusion''' ==<br />
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[[Category:Cloning]]</div>
Paul.xu