https://eln.excellgen.com/index.php?action=history&feed=atom&title=1554-pT7A3-MBP-i3C 1554-pT7A3-MBP-i3C - Revision history 2026-10-18T15:41:20Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1554-pT7A3-MBP-i3C&diff=8232&oldid=prev Paul.xu at 20:07, 14 February 2013 2013-02-14T20:07:03Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1554-pT7A3-MBP-i3C&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1025-pET28b-SM2 <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-1-10---2013-1-14 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1554&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1518+1554 pcr 1-7-2013.JPG]]&lt;br&gt; <br /> <br /> [[Image:1554 clone 1-10-2013.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 281px; height: 66px;&quot;<br /> |-<br /> | &lt;br&gt; <br /> | band 1&lt;br&gt; <br /> | band 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | PCR product&lt;br&gt; <br /> | 1429,pT7A3-MBP&lt;br&gt;<br /> |-<br /> | enzymes&lt;br&gt; <br /> | colspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; Nde1 + Xho1.&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-1-14 <br /> <br /> Make miniprep and digest with&amp;nbsp; Nde1 + Xho1. <br /> <br /> [[Image:42+1554+1600 digest detect 1-14-2013.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt;<br /> <br /> == Protein Expression[[1625 pT7A3-MBP-(3c)-KEAP1&amp;action=edit&amp;section=13|&lt;br&gt;]] ==<br /> <br /> [[1625 pCold-GST-(3c)-KEAP1#Protein_Expression|1625_pCold-GST-(3c)-KEAP1#Protein_Expression]] <br /> [[Category:Cloning]]</div> Paul.xu