https://eln.excellgen.com/index.php?action=history&feed=atom&title=1554_1613 1554 1613 - Revision history 2026-10-18T05:42:17Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1554_1613&diff=9969&oldid=prev Paul.xu at 18:49, 8 August 2013 2013-08-08T18:49:25Z <p></p> <p><b>New page</b></p><div>Data:2013-1-15---2013-1-18 <br /> <br /> Exprimenter: yingying <br /> <br /> 5 ml sample each , 1554,1613&amp;nbsp; induce with 0.3mM IPTG at&amp;nbsp; 25oC 0/N overnight&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 323px; height: 95px;&quot;<br /> |-<br /> | &#039;&#039;&#039;1554&#039;&#039;&#039; <br /> | pT7A3-MBP-i3C&lt;br&gt;<br /> |-<br /> | &#039;&#039;&#039;1613&#039;&#039;&#039; <br /> | pET28-cold-GST-3C&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |}<br /> <br /> {| width=&quot;200&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt; <br /> | 1&lt;br&gt; <br /> | 2&lt;br&gt; <br /> | 3&lt;br&gt; <br /> | 4&lt;br&gt;<br /> |-<br /> | clone ID&lt;br&gt; <br /> | colspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; 1554&lt;br&gt; <br /> | colspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; 1613&lt;br&gt;<br /> |-<br /> | competent cell&lt;br&gt; <br /> | BL21DE3pRare2&lt;br&gt; <br /> | BL21DE3pRare2pLysS&lt;br&gt; <br /> | BL21DE3pRare2&lt;br&gt; <br /> | BL21DE3pRare2pLysS&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> [[Image:1554+1613 protein screening 1-18-2013.jpg]]&lt;br&gt; <br /> <br /> &lt;br&gt; &lt;br&gt; <br /> <br /> &amp;nbsp;conclusion&amp;nbsp;: 1613,pET28-cold-GST-3C has expression (lane3,4) 47KD; 1554 expresses MBP&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Protein_Purification]]</div> Paul.xu