https://eln.excellgen.com/index.php?action=history&feed=atom&title=1560_pT7A3E-T4-Endo-IV 1560 pT7A3E-T4-Endo-IV - Revision history 2026-10-18T14:13:35Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1560_pT7A3E-T4-Endo-IV&diff=11889&oldid=prev Paul.xu at 14:55, 19 May 2014 2014-05-19T14:55:14Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1560 pT7A3E-T4-Endo-IV&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of1560 pT7A3E-T4-Endo-IV&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-2---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1560&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> Gene synthesis&lt;br&gt; <br /> <br /> [[Image:1560 2050pcr.JPG]]&lt;br&gt; <br /> <br /> digest with NdeI+XhoI&lt;br&gt; <br /> <br /> [[Image:2nd digest for 1560 2050.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014 <br /> <br /> Make miniprep, digest with NdeI+XhoI&lt;br&gt; <br /> <br /> [[Image:1560digest.JPG]]&lt;br&gt; <br /> <br /> SENT 1560-5 TO SEQUENCE,missing 1 G, can be fixed by PCR using primer&lt;br&gt;T4-Endo-IV-1 TAGCACCATATGCAGAAAACGAATCCTGGGTTACAGCGTCTGTTTCAGATTCCGACCT&lt;br&gt;T4-Endo-IV-14 GGTCGTACTCGAGAATGGAAAGATACCATCCGTTGTAGTTGCTTTTGC&lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Cloning of 1560 pT7A3E-T4-Endo-IV&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of1560 pT7A3E-T4-Endo-IV&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-2---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1560&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> pcr&lt;br&gt; <br /> <br /> [[Image:1560fixpcr 2062fixpcr2.JPG]]&lt;br&gt; <br /> <br /> digest with NdeI+XhoI&lt;br&gt; <br /> <br /> [[Image:Digest for 1560fix 2062fix.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014 <br /> <br /> Make miniprep, digest with NdeI+XhoI&lt;br&gt; <br /> <br /> &lt;br&gt; &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Cloning of 1560_pT7A3E-T4-Endo-I&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1560_pT7A3E-T4-Endo-I&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-5---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1560&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> subclone from 2132 to 1892 using Nde1 + Xho1 <br /> <br /> [[Image:Digest_for_2203_2205_1560.JPG]]&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014 <br /> <br /> Make miniprep, digest with Nde1 + Xho1 <br /> <br /> [[Image:1560_2203_2205digest.JPG]]&lt;br&gt; <br /> <br /> <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]<br /> <br /> <br /> <br /> <br /> [[Category:Cloning]]</div> Paul.xu