https://eln.excellgen.com/index.php?action=history&feed=atom&title=1568_pT7A3-Exo-VIII 1568 pT7A3-Exo-VIII - Revision history 2026-10-18T12:07:54Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1568_pT7A3-Exo-VIII&diff=7893&oldid=prev Paul.xu at 21:59, 18 January 2013 2013-01-18T21:59:09Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of&amp;nbsp;1568 pT7A3-Exo-VIII&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1568 pT7A3-Exo-VIII&lt;br&gt; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;December 31, 2012&lt;br&gt; <br /> <br /> &#039;&#039;&#039;Notebook:&amp;nbsp;&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID: 1568&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters: &#039;&#039;&#039;Jennifer Truong <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Cloning ===<br /> <br /> Date: December 31, 2012&lt;br&gt; <br /> <br /> Subclone from 1137 (insert lane one) p8H-Exo-VIII using Nde1 + Xho1 and vector 1278 (pT7A3) lane 2 <br /> <br /> Cut out fragments, ligate together and transform <br /> <br /> [[Image:1568 Digestion.JPG]] <br /> <br /> Date:&amp;nbsp;Jan 3, 2012&lt;br&gt; <br /> <br /> Subclone from 1137 (insert lane 5) p8H-Exo-VIII using Nde1 + Xho1 and vector 1514 (pT7A3-MBP-(3C)) lane 6&lt;br&gt; <br /> <br /> Cut out fragments, ligate together and transform &lt;br&gt; <br /> <br /> [[Image:1568 Digestion 1-3-12.JPG]]&lt;br&gt; <br /> <br /> === Screening ===<br /> <br /> Date:&amp;nbsp;Jan 2, 2013 <br /> <br /> Digested MiniPrep with RE Nde1 and Xho1 <br /> <br /> [[Image:1568 1569 1570 Screening.JPG]] <br /> <br /> See lanes 1-4 (samples 1-4) <br /> <br /> Insert wrong size&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039;&lt;br&gt; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Cloning of&amp;nbsp;1568 pT7A3-MBP-(3C)-Exo-VIII&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1568 pT7A3-MBP-(3C)-Exo-VIII <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;Jan, 2013&lt;br&gt; <br /> <br /> &#039;&#039;&#039;Notebook:&amp;nbsp;&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID: 1568&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters: &#039;&#039;&#039;Shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Cloning ===<br /> <br /> Subclone from 1137 (insert lane 5) p8H-Exo-VIII using Nde1 + Xho1 and vector 1514 (pT7A3-MBP-(3C)) lane 6&lt;br&gt; <br /> <br /> Cut out fragments, ligate together and transform &lt;br&gt; <br /> <br /> [[Image:1137_1514_1478_1533pcr_v12digest.JPG‎ ]]&lt;br&gt; <br /> <br /> === Screening ===<br /> <br /> Date:&amp;nbsp;Jan 2, 2013 <br /> <br /> Digested MiniPrep with RE Nde1 and Xho1 <br /> <br /> <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:1097_1127_1530_1279_1533_1568_1590digest.JPG]]<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039;&lt;br&gt; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu