https://eln.excellgen.com/index.php?action=history&feed=atom&title=1577_pT7A3E-RNaseR 1577 pT7A3E-RNaseR - Revision history 2026-10-18T11:23:25Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1577_pT7A3E-RNaseR&diff=12591&oldid=prev Excellgen: Created page with "== '''Cloning of 1577 pT7A3E-RNaseR''' == '''Purpose:''' Cloning of1577 pT7A3E-RNaseR1'''==''' '''Date: '''2014-7---- '''Notebook:0''' '''Page:''' '''Clone ID:1577''' ..." 2014-07-28T20:41:38Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 1577 pT7A3E-RNaseR&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of1577 pT7A3E-RNaseR1&#039;&#039;&#039;==&#039;&#039;&#039; &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-7---- &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone ID:1577&#039;&#039;&#039; ...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1577 pT7A3E-RNaseR&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of1577 pT7A3E-RNaseR1&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-7---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1577&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> PCR from MG1655 &lt;br&gt; <br /> <br /> [[Image:1577_1578_2238_1535_1855pcr.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; digest with Nde1 + XhoI, clone to 1892&lt;br&gt; <br /> <br /> [[Image:Digest_for_1577_1578_1535_1855.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014_7 <br /> <br /> Make miniprep, use Nde1 + XhoI to find positive clone&lt;br&gt; <br /> <br /> <br /> <br /> [[Image:1577_1578_2012_2049digest1.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Excellgen