https://eln.excellgen.com/index.php?action=history&feed=atom&title=1578_pT7A3-RecQ 1578 pT7A3-RecQ - Revision history 2026-10-18T11:55:24Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1578_pT7A3-RecQ&diff=12771&oldid=prev Excellgen at 18:51, 8 August 2014 2014-08-08T18:51:20Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1578 pT7A3-RecQR&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of1578 pT7A3-RecQ1&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-7---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1578&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> PCR from MG1655 &lt;br&gt; <br /> <br /> [[Image:1577 1578 2238 1535 1855pcr.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; digest with Nde1 + XhoI, clone to 1892&lt;br&gt; <br /> <br /> [[Image:Digest for 1577 1578 1535 1855.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014_7 <br /> <br /> Make miniprep, use Nde1 + XhoI to find positive clone&lt;br&gt; <br /> <br /> [[Image:1577 1578 2012 2049digest1.JPG]]&lt;br&gt; <br /> <br /> USE EcorRV to verify&lt;br&gt; <br /> <br /> [[Image:2238 1577 1578 2186 2272 2012digest.JPG]] <br /> <br /> sent 1578-4 to sequence, not right&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Cloning of 1578 pT7A3-RecQR&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of1578 pT7A3-RecQ1&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-8---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1578&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> PCR from MG1655 &lt;br&gt; <br /> <br /> [[Image:2256_2238_1578pcr.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; digest with Nde1 + XhoI, clone to 1892&lt;br&gt; <br /> <br /> [[Image:Digest_for_15782nd_2241_2213.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014_8 <br /> <br /> Make miniprep, use Nde1 + XhoI to find positive clone&lt;br&gt; <br /> <br /> [[Image:2012FIX_2303_1578_2166digest.JPG]]&lt;br&gt; <br /> <br /> <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Excellgen