Paul.xu at 20:16, 20 February 2013

2013-02-20T20:16:37Z

New page

== '''Cloning of 1584-pTriEx-TurboGFP''' ==

'''Purpose:''' Cloning of 1584-pTriEx-TurboGFP

'''Date: '''2013-1-10---2013-1-17

'''Notebook:0'''

'''Page:'''

'''Clone ID:1584'''

'''Experimenters:''' yingying

== '''Results''' ==

=== cloning ===

[[Image:1584 PCR 1-9-2013.JPG]] 

[[Image:1466+1543+1544+1584 clone 1-15-2013.JPG]] 

 

digest PCR product with BspHI + Xho1, ligate to Nco1 + Xho1 digested vector 1434 pTriEX

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=== Colony Screening ===

Date: 2013-1-17

Make miniprep and digest with Nco1

[[Image:1584 digest detect 1-17-2013.JPG]]

 

== '''Result intepretation/Summary''' ==

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== '''Conclusion''' ==

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== '''Cloning of 1584-pTriEx-TurboGFP''' ==

'''Purpose:''' Cloning of 1584-pTriEx-TurboGFP

'''Date: '''2013-2-4----2013-2-7

'''Notebook:0'''

'''Page:'''

'''Clone ID:1584'''

'''Experimenters:''' yingying

== '''Results''' ==

=== cloning ===

T7-BspHI-Nhe1-TurboGFP-F2

TurboGFP-Xho1-R 

[[Image:1584 pcr 2-4-2013.JPG]] 

 

 

digest PCR product with BspHI + Xho1, ligate to Nco1 + Xho1 digested vector 1434 pTriEX

[[Image:1584+1656+1624 clone 2-5-2013.JPG]]

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=== Colony Screening ===

Date: 2013-2-7

Make miniprep and digest with Nco1

[[Image:1584+1624 digest detect 2-7-2013.JPG]] 

 

== '''Result intepretation/Summary''' ==

*&amp;lt;br&amp;gt;

== '''Conclusion''' ==

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== '''Cloning of 1584-pTriEx-TurboGFP''' ==

'''Purpose:''' Cloning of 1584-pTriEx-TurboGFP

'''Date: '''2013-2-14---2013-2-19

'''Notebook:0'''

'''Page:'''

'''Clone ID:1584'''

'''Experimenters:''' yingying

== '''Results''' ==

=== cloning ===

T7-BspHI-Nhe1-TurboGFP-F3

TurboGFP-Xho1-R 

[[Image:1584+1657 pcr 2-14-2013.JPG]] 

 

 

digest PCR product with BspHI + Xho1, ligate to Nco1 + Xho1 digested vector 1434 pTriEX

[[Image:1584+1674 clone 2-15-2013.JPG]] 

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=== Colony Screening ===

Date: 2013-2-19

Make miniprep and digest with Nco1

[[Image:1540+1584_digest_detct_2-19-2013.JPG]] 

 

== '''Result intepretation/Summary''' ==

*&amp;lt;br&amp;gt;

== '''Conclusion''' ==

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[[Category:Cloning]]

1584-pTriEx-TurboGFP - Revision history

Paul.xu at 20:16, 20 February 2013