https://eln.excellgen.com/index.php?action=history&feed=atom&title=1588_pT7A3-MBP-%283c%29-Exo-VII 1588 pT7A3-MBP-(3c)-Exo-VII - Revision history 2026-10-18T12:30:56Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1588_pT7A3-MBP-(3c)-Exo-VII&diff=8827&oldid=prev Paul.xu at 18:29, 8 April 2013 2013-04-08T18:29:33Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of ‎1588 pT7A3-MBP-(3c)-Exo-VII&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1588 pT7A3-MBP-(3c)-Exo-VII <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-3-7--- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1588&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; Shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039;&lt;br&gt; ==<br /> <br /> === PCR ===<br /> <br /> PCR from genomic DNA isolated from Escherichia coli str. K-12 substr. MG1655 <br /> <br /> [[Image:1588 1592PCR.JPG]]&lt;br&gt; <br /> <br /> Cut out the band, digest PCR products and&amp;nbsp;1514 pT7A3-MBP-(3C) with Nde1 + Xho1&lt;br&gt; <br /> <br /> [[Image:Digest for1588.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013- <br /> <br /> Make miniprep and digest withNde1 + Xho1 <br /> <br /> [[Image:1588digest.JPG]]&lt;br&gt; <br /> <br /> send 1588-6 to sequence, missing 1 C&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Cloning of ‎1588 pEC-Exo-VII&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1588 pEC-Exo-VII <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-4---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1588&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; Shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039;&lt;br&gt; ==<br /> <br /> === PCR ===<br /> <br /> PCR from genomic DNA isolated from Escherichia coli str. K-12 substr. MG1655 <br /> <br /> [[Image:1588pcr 03 27 2013.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> Cut out the band, digest PCR products and&amp;nbsp;1454 with Nde1 + Xho1&lt;br&gt; <br /> <br /> [[Image:IMG 5745.JPG]]&lt;br&gt; &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013- <br /> <br /> Make miniprep and digest withNde1 + Xho1 <br /> <br /> [[Image:1588 1592pecdigest.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu