https://eln.excellgen.com/index.php?action=history&feed=atom&title=1592-pT7A3-Ecoli-Topo_I 1592-pT7A3-Ecoli-Topo I - Revision history 2026-10-18T14:43:33Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1592-pT7A3-Ecoli-Topo_I&diff=10950&oldid=prev Paul.xu: Created page with "== '''Cloning of 1592-pT7A3-Ecoli-Topo I''' == '''Purpose:''' Cloning of 1592-pT7A3-Ecoli-Topo I '''Date: '''2014-1-6---2014-1-10 '''Notebook:0''' '''Page:''' '''Clone ID..." 2014-01-14T21:26:50Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 1592-pT7A3-Ecoli-Topo I&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1592-pT7A3-Ecoli-Topo I &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-1-6---2014-1-10 &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone ID...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1592-pT7A3-Ecoli-Topo I&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1592-pT7A3-Ecoli-Topo I<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-1-6---2014-1-10<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1592&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:715_718_1592clone_1-8-2014.JPG]]<br /> <br /> {| width=&quot;200&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | LANE&lt;br&gt;<br /> | 5&lt;br&gt;<br /> | 6&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | PCR &lt;br&gt;<br /> | 1279 pT7A3&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | colspan=&quot;2&quot; | Nde1 + Xho1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-1-10<br /> <br /> Make miniprep and digest with&amp;amp;nbsp; Nde1 + Xho1 <br /> <br /> [[Image:1315_1592digest_1-10-2014.JPG]]&lt;br&gt; <br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; [[Category:Cloning]]</div> Paul.xu