https://eln.excellgen.com/index.php?action=history&feed=atom&title=1592_pT7A3-MBP-%283c%29-Ecoli-Topo_I 1592 pT7A3-MBP-(3c)-Ecoli-Topo I - Revision history 2026-10-18T05:37:22Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1592_pT7A3-MBP-(3c)-Ecoli-Topo_I&diff=8828&oldid=prev Paul.xu at 18:32, 8 April 2013 2013-04-08T18:32:01Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of ‎1592 pT7A3-MBP-(3c)-Ecoli-Topo I&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1592 pT7A3-MBP-(3c)-Ecoli-Topo I <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-2-7--- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1592&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; Shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039;&lt;br&gt; ==<br /> <br /> === PCR ===<br /> <br /> PCR from genomic DNA isolated from Escherichia coli str. K-12 substr. MG1655 &lt;br&gt; <br /> <br /> USE 5ul MG1655 genomic DNA as template&lt;br&gt; <br /> <br /> [[Image:1588 1592PCR.JPG]]&lt;br&gt; <br /> <br /> Cut out the band, digest PCR products and&amp;nbsp;1514 pT7A3-MBP-(3C) with Nde1 + Xho1&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:Digest for1535 1426 1592.JPG]]&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013- <br /> <br /> Make miniprep and digest withNde1 + Xho1 <br /> <br /> [[Image:1048 1144 1592digest.JPG]] <br /> <br /> &amp;lt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Cloning of ‎1592 pEC-Ecoli-Topo I&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1592 pEC-Ecoli-Topo I <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-4-7--- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1592&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; Shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039;&lt;br&gt; ==<br /> <br /> === PCR ===<br /> <br /> PCR from genomic DNA isolated from Escherichia coli str. K-12 substr. MG1655 &lt;br&gt; <br /> <br /> USE 5ul MG1655 genomic DNA as template&lt;br&gt; <br /> <br /> [[Image:1265_1592pcr_03_27_2013.JPG]]<br /> <br /> Cut out the band, digest PCR products and&amp;nbsp;1454 pT7A3-MBP-(3C) with Nde1 + Xho1&lt;br&gt; <br /> <br /> [[Image:IMG 5745.JPG]]&lt;br&gt; &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013- <br /> <br /> Make miniprep and digest with Nde1 + Xho1+BamHI <br /> <br /> [[Image:1588 1592pecdigest.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> =<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> <br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> <br /> <br /> [[Category:Cloning]]</div> Paul.xu