https://eln.excellgen.com/index.php?action=history&feed=atom&title=1597-pT7A3-6XHis.GST 1597-pT7A3-6XHis.GST - Revision history 2026-10-18T20:58:33Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1597-pT7A3-6XHis.GST&diff=7790&oldid=prev Paul.xu: Created page with "== '''Cloning of 1597-pT7A3-6XHis.GST''' == '''Purpose:''' Cloning of 1597-pT7A3-6XHis.GST '''Date: '''2013-1-7---2013-1-9 '''Notebook:0''' '''Page:''' '''Clone ID:1597''..." 2013-01-09T16:54:41Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 1597-pT7A3-6XHis.GST&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1597-pT7A3-6XHis.GST &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-1-7---2013-1-9 &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone ID:1597&#039;&#039;...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1597-pT7A3-6XHis.GST&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1597-pT7A3-6XHis.GST<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-1-7---2013-1-9<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1597&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === subcloning ===<br /> <br /> [[Image:1597_clone_1-7-2013.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 281px; height: 66px;&quot;<br /> |-<br /> | &lt;br&gt; <br /> | band 1&lt;br&gt; <br /> | band 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | pDB.His.GST&lt;br&gt; <br /> | pT7A3-MBP-(3C)&lt;br&gt;<br /> |-<br /> | enzymes&lt;br&gt; <br /> | colspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; Nco1 + Xho1.&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-1-9 <br /> <br /> Make miniprep and digest with Nco1 + Xho1. <br /> <br /> [[Image:1597_digest_detect_1-9-2013.JPG]]&lt;br&gt; <br /> <br /> clone 1,2,3,4,5,6 right<br /> <br /> c &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&amp;amp;lt;br&amp;amp;gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &amp;amp;lt;br&amp;amp;gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &amp;amp;lt;br&amp;amp;gt; <br /> <br /> {| cellspacing=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> {| cellspacing=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Category:Cloning]]</div> Paul.xu